Variable microsatellite loci isolated from the Asian honeybee, Apis cerana (Hymenoptera; Apidae)

被引：18

作者：

Takahashi, Jun-Ichi ^{[1
,2
]}

Shimizu, Souta ^{[2
]}

Koyama, Satoshi ^{[3
]}

Kimura, Kiyoshi ^{[4
]}

Shimizu, Isamu ^{[1
]}

Yoshida, Tadaharu ^{[2
]}

机构：

[1] Kyoto Univ, Ctr Ecol Res, Shiga 5202113, Japan

[2] Tamagawa Univ, Honeybee Sci Res Ctr, Res Inst, Tokyo 1948610, Japan

[3] Tokyo Univ Agr & Technol, Dept Vet Med, Fac Agr, Fuchu, Tokyo 1838509, Japan

[4] Natl Agr & Food Res Org, Natl Inst Livestock & Grassland Sci, Anim Breeding & Reprod Res Team, Tsukuba, Ibaraki 3050901, Japan

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 03期

关键词：

Apis cerana; honeybee; mating number; microsatellite DNA; relatedness; MELLIFERA L; BEE;

D O I：

10.1111/j.1755-0998.2009.02268.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed 12 polymorphic microsatellite loci for the Asian honeybee, Apis cerana using the magnetic particle method. Eight of these 12 were highly polymorphic, having four to seven alleles with an expected heterozygosity of 0.38 to 0.78. The primers also produce polymorphic products in related honeybee species such as Apis nigrocincta. These loci can be used to study parameters associated with genetic structure, such as paternity frequency and worker reproduction.

引用

页码：819 / 821

页数：3

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