Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization- a correlative study with urine PCA3 and TMPRSS2-ERG

被引：26

作者：

Warrick, Joshua I. ^{[1
]}

Tomlins, Scott A. ^{[1
,2
,3
,4
]}

Carskadon, Shannon L. ^{[1
,2
]}

Young, Allison M. ^{[1
]}

Siddiqui, Javed ^{[1
,2
]}

Wei, John T. ^{[4
]}

Chinnaiyan, Arul M. ^{[1
,2
,3
,4
,5
]}

Kunju, Lakshmi P. ^{[1
,2
,3
]}

Palanisamy, Nallasivam ^{[1
,2
,3
]}

机构：

[1] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48104 USA

[2] Univ Michigan, Sch Med, Michigan Ctr Translat Pathol, Ann Arbor, MI USA

[3] Univ Michigan, Sch Med, Ctr Comprehens Canc, Ann Arbor, MI USA

[4] Univ Michigan, Sch Med, Dept Urol, Ann Arbor, MI USA

[5] Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA

来源：

MODERN PATHOLOGY | 2014年 / 27卷 / 04期

基金：

美国国家卫生研究院;

关键词：

PCA3; prostate cancer; RNA in situ hybridization; ANTIBODY-BASED DETECTION; ETS GENE FUSIONS; INTRAEPITHELIAL NEOPLASIA; ANTIGEN; REARRANGEMENT STATUS; TMPRSS2/ERG; CARCINOMA; DD3;

D O I：

10.1038/modpathol.2013.169

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.

引用

页码：609 / 620

页数：12

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