Inflammatory Microenvironment Changes the Secretory Profile of Mesenchymal Stem Cells to Recruit Mesenchymal Stem Cells

被引:18
|
作者
Xing, Junchao [1 ,2 ,3 ,4 ]
Hou, Tianyong [1 ,2 ,3 ,4 ]
Jin, Huiyong [1 ,2 ,3 ,4 ]
Luo, Fei [1 ,2 ,3 ,4 ]
Chang, Zhengqi [1 ,2 ,3 ,4 ]
Li, Zhiqiang [1 ,2 ,3 ,4 ]
Xie, Zhao [1 ,2 ,3 ,4 ]
Xu, Jianzhong [1 ,2 ,3 ,4 ]
机构
[1] Third Mil Med Univ, Natl & Local United Engn Lab Tissue Engn, Dept Orthopaed, Southwest Hosp, Chongqing, Peoples R China
[2] Third Mil Med Univ, Ctr Tissue Engn Res & Applicat, Chongqing, Peoples R China
[3] Lab Tissue Engn Chongqing City, Chongqing, Peoples R China
[4] Ctr Regenet & Reconstruct Engn Technol Chongqing, Chongqing, Peoples R China
基金
中国国家自然科学基金;
关键词
Mesenchymal stem cells; Cell migration; Inflammatory microenvironment; Chemokine secretion; MARROW STROMAL CELLS; MYOCARDIAL-INFARCTION; CHEMOKINE RECEPTORS; TISSUE REGENERATION; CXC CHEMOKINES; GROWTH; REPAIR; MIGRATION; ALPHA; TRANSDIFFERENTIATION;
D O I
10.1159/000358663
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background/Aims: Human bone-marrow mesenchymal stem cells (hBMSCs) are widely transplanted into inflammatory microenvironment to accelerate tissue regeneration. Transplanted hBMSCs recruit host hBMSCs through a poorly understood mechanism. This study was aimed to determine whether and how inflammatory microenvironment influenced the host-hBMSCs-recruiting capability of transplanted hBMSCs. Methods: Pro-inflammatory factors, including IL-1 beta, IL-6 and TNF-alpha, were utilized to mimic inflammatory microenvironment. hBMSCs were cultured and conditioned media (CM) were collected. The effects of inflammatory microenvironment on the host-hBMSCs-recruting capability of cultured hBMSCs were revealed by transwell migration assays. Employing semi-quantitative and quantitative cytokine antibody assays, we examined the secretory profile of cultured hBMSCs. Results: CM from cultured hBMSCs exerted excellent host-hBMSCs-recruting capability, which was significantly promoted by exposure to inflammatory microenvironment. Within inflammatory microenvironment, hBMSCs secreted more chemokines related to cell migration. Finally, 21 cytokines were verified as potential factors accounting for the enhanced host-hBMSCs-recruting capability of cultured hBMSCs exposed to inflammatory microenvironment. Conclusion: These results strongly suggested that in clinic, inflammatory microenvironment might promote the host-hBMSCs-recruiting capacity of transplanted hBMSCs by increasing chemokines secretion. Modulation of such characteristics of hBMSCs might provide novel therapeutic ideas in the context of hBMSCs. Copyright (C) 2014 S. Karger AG, Basel
引用
收藏
页码:905 / 919
页数:15
相关论文
共 50 条
  • [1] Mesenchymal stem cells and their microenvironment
    Liu, Jiaxi
    Gao, Jinfang
    Liang, Zixie
    Gao, Chong
    Niu, Qing
    Wu, Fengping
    Zhang, Liyun
    [J]. STEM CELL RESEARCH & THERAPY, 2022, 13 (01)
  • [2] Mesenchymal stem cells and their microenvironment
    Schraufstatter, Ingrid U.
    Discipio, Richard G.
    Khaldoyanidi, Sophia
    [J]. FRONTIERS IN BIOSCIENCE-LANDMARK, 2011, 16 : 2271 - 2288
  • [3] Mesenchymal stem cells and their microenvironment
    Jiaxi Liu
    Jinfang Gao
    Zixie Liang
    Chong Gao
    Qing Niu
    Fengping Wu
    Liyun Zhang
    [J]. Stem Cell Research & Therapy, 13
  • [4] Mesenchymal stem cells as a vector for the inflammatory prostate microenvironment
    Brennen, W. Nathaniel
    Denmeade, Samuel R.
    Isaacs, John T.
    [J]. ENDOCRINE-RELATED CANCER, 2013, 20 (05) : R269 - R290
  • [5] The roles of mesenchymal stem cells in tumor inflammatory microenvironment
    Sun, Zhao
    Wang, Shihua
    Zhao, Robert Chunhua
    [J]. JOURNAL OF HEMATOLOGY & ONCOLOGY, 2014, 7
  • [6] The roles of mesenchymal stem cells in tumor inflammatory microenvironment
    Zhao Sun
    Shihua Wang
    Robert Chunhua Zhao
    [J]. Journal of Hematology & Oncology, 7
  • [7] Mesenchymal Stem Cells in the Tumor Microenvironment
    Atiya, Huda
    Frisbie, Leonard
    Pressimone, Catherine
    Coffman, Lan
    [J]. TUMOR MICROENVIRONMENT: NON-HEMATOPOIETIC CELLS, 2020, 1234 : 31 - 42
  • [8] Autophagy promotes apoptosis of mesenchymal stem cells under inflammatory microenvironment
    Shipeng Dang
    Zhi-ming Yu
    Chang-ying Zhang
    Jie Zheng
    Ku-lin Li
    Ying Wu
    Ling-ling Qian
    Zhen-yu Yang
    Xiao-rong Li
    Yanyun Zhang
    Ru-xing Wang
    [J]. Stem Cell Research & Therapy, 6
  • [9] Autophagy promotes apoptosis of mesenchymal stem cells under inflammatory microenvironment
    Dang, Shipeng
    Yu, Zhi-ming
    Zhang, Chang-ying
    Zheng, Jie
    Li, Ku-lin
    Wu, Ying
    Qian, Ling-ling
    Yang, Zhen-yu
    Li, Xiao-rong
    Zhang, Yanyun
    Wang, Ru-xing
    [J]. STEM CELL RESEARCH & THERAPY, 2015, 6
  • [10] Mesenchymal Stem Cells: Their Role in the Tumor Microenvironment
    Idarraga, Jhon Alexander
    Munera, Luz Marina Restrepo
    [J]. TISSUE ENGINEERING PART B-REVIEWS, 2023, 29 (06) : 681 - 691