High-throughput measurement of the enantiomeric excess of chiral alcohols by using two enzymes

被引：31
|
作者：
Li, Z [1 ]
Bütikofer, L [1 ]
Witholt, B [1 ]
机构：
[1] ETH Honggerberg, Inst Biotechnol, CH-8093 Zurich, Switzerland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 13期
关键词：
alcohols; asymmetric catalysis; dehydrogenation; enantioselectivity; high-throughput screening;
D O I：
10.1002/anie.200353055
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Rapid ee determination: Enantioselective alcohol dehydrogenases A and B were used to oxidize chiral alcohols in a sensitive, accurate, high-throughput method (see scheme). The reaction rates were determined by monitoring the formation of NAD(P)H by UV spectroscopy. The ee value was calculated from the reaction rates and the kinetic constants of the enzymes.
引用
收藏
页码：1698 / 1702
页数：5
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