rSJYB1 inhibits collagen type I protein expression in hepatic stellate cells via down-regulating activity of collagen α1 (I) promoter

被引：2
|
作者：
Chen, Liuting [1 ]
Ji, Zhaodong [2 ,3 ]
Duan, Lian [4 ]
Zhu, Dandan [1 ]
Chen, Jinling [1 ]
Sun, Xiaolei [1 ]
Yu, Yang [1 ]
Duan, Yinong [1 ]
机构：
[1] Nantong Univ, Sch Med, Dept Pathogen Biol, Nantong, Jiangsu, Peoples R China
[2] Fudan Univ, Canc Inst, Shanghai Canc Ctr, Shanghai, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai, Peoples R China
[4] Nantong Univ, Sch Med, Dept Med Informat, Nantong, Jiangsu, Peoples R China
来源：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2019年 / 23卷 / 05期
基金：
中国国家自然科学基金;
关键词：
collagen type I; hepatic stellate cell; liver fibrosis; SJYB1; SOLUBLE EGG ANTIGENS; BOX-BINDING-PROTEIN; SCHISTOSOMA-MANSONI; ACTIVATION; TRANSCRIPTION; APOPTOSIS; YB-1; DNA;
D O I：
10.1111/jcmm.14271
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
YB1 is a negative regulator in liver fibrosis. We wondered whether SJYB1, a homologous protein of YB1 from Schistosoma japonicum, has an effect on liver fibrosis in vitro. Recombinant SJYB1 (rSJYB1) protein was expressed in a bacterial system and purified by Ni-NTA His-Bind Resin. A human hepatic stellate cell line, the LX-2 cell line, was cultured and treated with rSJYB1. The role of rSJYB1 on LX-2 cells was then analysed by Western blot and luciferase assay. We succeeded in expressing and purifying SJYB1 in a bacterial system and the purified rSJYB1 could be recognized by S japonicum-infected rabbit sera. Western bolt analysis showed that rSJYB1 inhibited the expression of collagen type I, but had little effect on alpha-smooth muscle actin (alpha-SMA). Further analysis revealed that rSJYB1 inhibited the activity of collagen alpha 1 (I) (COL1A1) promoter and functioned at -1592/-1176 region of COL1A1 promoter. Our data demonstrate that rSJYB1-mediated anti-fibrotic activity involves inhibiting the activity of COL1A1 promoter and subsequently suppressing the expression of collagen type I in hepatic stellate cells.
引用
收藏
页码：3676 / 3682
页数：7
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