Micron Polarization-sensitive Spectral-domain Optical Coherence Tomography Based on Single Camera

被引:2
|
作者
Qiu Zhiyuan [1 ]
Gao Wanrong [1 ]
Chen Chaoliang [2 ]
Chang Ying [1 ]
机构
[1] Nanjing Univ Sci & Technol, Sch Elect Engn & Optoelect Tech, Nanjing 210094, Peoples R China
[2] Southeast Univ, Sch Elect Sci & Engn, Nanjing 210096, Peoples R China
基金
中国国家自然科学基金;
关键词
Biomedical imaging; Polarization; sensitive optical coherence tomography; Single camera; Dispersion compensation; ULTRAHIGH-RESOLUTION; HIGH-SPEED; DISPERSION; LINE; FIBER;
D O I
10.3788/gzxb20225112.1217002
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Optical Coherence Tomography(OCT) is an interferometric imaging method,and it is mostly used in the field of imaging for its non- invasive, high-resolution and high- speed properties. This technology can also be applied to detect defects in materials. Although OCT can provide images of morphological structure,it can not distinguish tissues with similar light intensity properties in pathologies. Polarization- sensitive Optical Coherence Tomography (PS- OCT) is an imaging system extended from conventional OCT,enabling functional imaging. It can use Stokes parameters,Jones and Muller matrices to calculate the polarization properties of the samples,like the birefringence phase retardation,the optic axis orientation and depolarization. PS- OCT has been used in a number of medical applications,such as burn depth determination and tumor yield assessment. And it also can be applied to examination of stressinduced birefringence of materials. In previous systems, it is required to detect the two orthogonally polarized components by using dual cameras based spectrometers. But there are problems with this system arrangement. For example, it has high cost and requires complex hardware and software designs. In addition, it is hard to achieve the uniform detection for two cameras,the mismatch between the two channels can lead to polarization distortions and failure to calculate the true information and additional algorithms are necessary to tackle it. So a series of single cameras based methods have been proposed. Single cameras based systems can achieve time-sharing detection or real- time detection of two orthogonal channels with relatively lower cost and simpler system setup. Wollaston prism,optical switch,grating and multi- camera are often used to achieve single camera detection. Improving the axial resolution of the system can enable it to have more potential applications. The OCT system with micron axial resolution can achieve cellular and subcellular level imaging and detect subsurface defects in ceramics or other materials. In order to achieve such a high axial resolution,super-continuum light source is generally used to increase the bandwidth of imaging. Because the optical path of the reference arm and the sample arm are not completely symmetrical,as the spectral bandwidth increases,the system can introduce serious dispersion and affect axial resolution. In this paper,we demonstrate a polarization- sensitive spectral domain optical coherence tomography imaging system using a single camera with micron axial resolution. It is an all single-mode fiber-based system,and a broad bandwidth light source is used to achieve micron axial resolution. In order to increase actual axial resolution of the system,the system dispersion effects are compensated by using both hardware and software methods. After compensating the first order and second order dispersion, the measured axial resolution of the system is about 1.61 mu m for the sample with an approximated refractive index of 1.4. In order to realize the measurement of the polarization state of the light reflected from the sample,the polarization state of light incident on the sample surface and the reference arm is modulated by the polarizer and four polarization controllers. The horizontal and vertical polarization interference signals are separately measured via channel switching of a polarizer and they can achieve time-sharing detection by using only one camera. Intensity and phase retardation information of the samples can be calculated by the signals obtained from the two channels at different times. To verify the capability of our system to measure the polarization information,we succeed in polarization imaging with a single camera and obtaining the images of intensity and polarization parameter contrast of the biological tissue in vitro by using Stokes vector. From the retardation image of bovine tendon at different position,it can be obviously observed that the phase retardation varies periodically with the increase of the depth in the tissue. This method is characterized by its simple system arrangement and lay the basis for miniaturizing in vivo high resolution polarization parameter imaging.
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页数:10
相关论文
共 35 条
  • [1] In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography
    An, Lin
    Wang, Ruikang K.
    [J]. OPTICS EXPRESS, 2008, 16 (15) : 11438 - 11452
  • [2] Blind dispersion compensation for optical coherence tomography
    Banaszek, Konrad
    Radunsky, Aleksandr S.
    Walmsley, Ian A.
    [J]. OPTICS COMMUNICATIONS, 2007, 269 (01) : 152 - 155
  • [3] Single camera based spectral domain polarization sensitive optical coherence tomography
    Baumann, Bernhard
    Goetzinger, Erich
    Pircher, Michael
    Hitzenberger, Christoph K.
    [J]. OPTICS EXPRESS, 2007, 15 (03): : 1054 - 1063
  • [4] Optical coherence tomography retinal ganglion cell complex analysis for the detection of early chiasmal compression
    Blanch, Richard J.
    Micieli, Jonathan A.
    Oyesiku, Nelson M.
    Newman, Nancy J.
    Biousse, Valerie
    [J]. PITUITARY, 2018, 21 (05) : 515 - 523
  • [5] Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography
    Cense, B
    Nassif, NA
    Chen, T
    Pierce, M
    Yun, SH
    Park, BH
    Bouma, BE
    Tearney, GJ
    de Boer, JF
    [J]. OPTICS EXPRESS, 2004, 12 (11): : 2435 - 2447
  • [6] Polarization-sensitive spectral-domain optical coherence tomography using a single line scan camera
    Cense, Barry
    Mujat, Mircea
    Chen, Teresa C.
    Park, B. Hyle
    de Boer, Johannes F.
    [J]. OPTICS EXPRESS, 2007, 15 (05): : 2421 - 2431
  • [7] CHEN C L, 2022, CHIN OPT LETT, V20, P1
  • [8] Dual spectrometer system with spectral compounding for 1-μm optical coherence tomography in vivo
    Cui, Dongyao
    Liu, Xinyu
    Zhang, Jing
    Yu, Xiaojun
    Ding, Sun
    Luo, Yuemei
    Gu, Jun
    Shum, Ping
    Liu, Linbo
    [J]. OPTICS LETTERS, 2014, 39 (23) : 6727 - 6730
  • [9] Polarization sensitive optical coherence tomography - a review [Invited]
    de Boer, Johannes F.
    Hitzenberger, Christoph K.
    Yasuno, Yoshiaki
    [J]. BIOMEDICAL OPTICS EXPRESS, 2017, 8 (03): : 1838 - 1873
  • [10] Two-dimensional birefringence imaging in biological tissue by polarization-sensitive optical coherence tomography
    deBoer, JF
    Milner, TE
    vanGemert, MJC
    Nelson, JS
    [J]. OPTICS LETTERS, 1997, 22 (12) : 934 - 936