Synthesis, Pharmacological, and Biological Screening of Novel Derivatives of Benzodiazepines

A series of novel 4-(substituted phenyl)-2-(thiophen-2-yl)-2,3-dihydro-1H-benzo[b][1,4]diazepine have been synthesized from 3-(substituted phenyl)-1-(thiophen-2-yl)prop-2-en-1-one. 3-(Substituted phenyl)-1-(thiophen-2-yl)prop-2-en-1-one was prepared by condensing 2-acetyl thiophene with various aromatic aldehydes in the presence of 20% NaOH as base. Different 3-(substituted phenyl)-1-(thiophen-2-yl)prop-2-en-1-one on cyclization with o-phenylenediamine in the presence of NaOH as base resulted in 4-(substituted phenyl)-2-(thiophen-2-yl)-2,3-dihydro-1H-benzo[b][1,4]diazepine derivatives. The structures of synthesized compounds are confirmed by IR, H-1 NMR, mass spectra, and elemental analysis. All the compounds have been screened for their antimicrobial, analgesic, and anti-inflammatory activities.