Versatile two-phase solvent system for flavonoid prefractionation by high-speed countercurrent chromatography

被引：0
|
作者：
Yuan, LM [1 ]
Ai, P [1 ]
Chen, XX [1 ]
Zi, M [1 ]
Wu, P [1 ]
Li, ZY [1 ]
Chen, YG [1 ]
机构：
[1] Yunnan Normal Univ, Dept Chem, Kunming 650092, Peoples R China
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2002年 / 25卷 / 06期
关键词：
D O I：
10.1081/JLC-120005949
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed countercurrent chromatography was used for the prefractionation of flavonoids from the crude extracts of Pericarpium citri reticulatae, Radix puerariae, Radix glycyrrhizae, Radix scutellariae, and Flos genkwa. All separations were performed only with a two-phase system composed Of CHCl3: CH3OH: H2O=4:x:2. The x was changed for each sample between 2.5 similar to 4.5. The fractionated components were identified by thin-layer chromatography, which confirmed this solvent system was versatile and very useful for prefractionation of flavonoids.
引用
收藏
页码：889 / 897
页数：9
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