A 2H NMR study of macroscopically aligned bilayer membranes containing interfacial hydroxyl residues

被引:35
|
作者
Kurze, V [1 ]
Steinbauer, B [1 ]
Huber, T [1 ]
Beyer, K [1 ]
机构
[1] Univ Munich, Adolf Butenandt Inst, Lehrstuhl Stoffwechselbiochem, D-80336 Munich, Germany
关键词
D O I
10.1016/S0006-3495(00)76788-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The polar interface of membranes containing phosphatidylglycerol or cholesterol was studied by H-2 nuclear magnetic resonance (NMR) as a function of membrane hydration. The membranes were macroscopically aligned and hydrated with deuterium oxide. Water uptake and membrane annealing was achieved under NMR control, using a novel hydration technique. Well-resolved H-2 quadrupolar doublets were obtained from individual hydroxyl residues and from the interlamellar water. The response of the phosphatidylglycerol headgroup and of the cholesterol molecule to the spontaneous evaporation of interlamellar water could be thus monitored continuously. It is shown that the phosphatidylglycerol headgroup undergoes changes of conformation and average orientation with respect to the membrane surface and that the off-axis motion of the cholesterol molecule decreases. The deuteron exchange between hydroxyl residues and surface-associated D2O was determined by an inversion transfer technique. The exchange rates of the hydroxyl residues in the phosphatidylglycerol headgroup were different and depended strongly on the total hydration of the membrane. Significantly lower and almost hydration-independent rates were obtained for cholesterol. These results will be discussed with reference to earlier reports on the headgroup dynamics of phosphatidylglycerol and on the interaction of cholesterol with the membrane-water interface.
引用
收藏
页码:2441 / 2451
页数:11
相关论文
共 50 条
  • [1] Interaction of protoporphyrin IX with magnetically aligned phospholipid bilayer membranes:: A 2H NMR study.
    Prosser, RS
    Shiyanovskaya, IV
    Malmer, M
    Westerman, PW
    [J]. BIOPHYSICAL JOURNAL, 2001, 80 (01) : 369A - 369A
  • [2] Solid-State 2H NMR spectroscopy of retinal proteins in aligned membranes
    Brown, Michael F.
    Heyn, Maarten P.
    Job, ConstAntin
    Kim, Suhkmann
    Moltke, Stephan
    Nakanishi, Koji
    Nevzorov, Alexander A.
    Struts, Andrey V.
    Salgado, Gilmar F. J.
    Wallat, Ingrid
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (12): : 2979 - 3000
  • [3] Molecular dynamics simulations and 2H NMR study of the GalCer/DPPG lipid bilayer
    Zaraiskaya, T
    Jeffrey, KR
    [J]. BIOPHYSICAL JOURNAL, 2005, 88 (06) : 4017 - 4031
  • [4] Solid state 2H NMR study of 'lipid raft' model membranes
    Shaikh, SR
    Brzustowicz, M
    Stillwell, W
    Wassall, S
    [J]. BIOPHYSICAL JOURNAL, 2002, 82 (01) : 153A - 153A
  • [5] Identifying different hydroxyl populations in cellulose by 2H MAS NMR
    Lindh, Erik
    Terenzi, Camilla
    Furo, Istvan
    Salmen, Lennart
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 249
  • [6] THE INTERACTION OF ALCOHOLS WITH LIPID BILAYER-MEMBRANES - A H-2-NMR STUDY
    PHONPHOK, N
    WESTERMAN, PW
    POPE, JM
    DOANE, JW
    DUBRO, DW
    [J]. BIOPHYSICAL JOURNAL, 1988, 53 (02) : A498 - A498
  • [7] Structural analysis and dynamics of retinal chromophore in dark and metal states of rhodopsin from 2H NMR of aligned membranes
    Struts, Andrey V.
    Salgado, Gilmar F. J.
    Tanaka, Katsunori
    Krane, Sonja
    Nakanishi, Koji
    Brown, Michael F.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2007, 372 (01) : 50 - 66
  • [8] Hydration study of homopolypeptides by 2H NMR
    Peemoeller, H.
    Stanley, J. A.
    MacMillan, M. B.
    Weglarz, W. P.
    Bennett, J. C.
    Corbett, J. M.
    Hawton, M.
    Holly, R.
    [J]. BIOPOLYMERS, 2007, 86 (01) : 11 - 22
  • [9] The EGF receptor transmembrane domain:: 2H NMR study of peptide phosphorylation effects in a bilayer environment
    Jones, DH
    Barber, KR
    Grant, CWM
    [J]. BIOCHEMISTRY, 1998, 37 (20) : 7504 - 7508
  • [10] Sites of hydroxyl radical reaction with amino acids identified by 2H NMR detection of induced 1H/2H exchange
    Nukuna, BN
    Goshe, MB
    Anderson, VE
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (06) : 1208 - 1214