MicroRNA-218 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and Accelerates Bone Fracture Healing

被引：36
|
作者：
Shi, Liu [1 ,2 ]
Feng, Lu [1 ,2 ]
Liu, Yang [1 ,2 ]
Duan, Ji-qiang [3 ]
Lin, Wei-ping [1 ,2 ]
Zhang, Jin-fang [4 ]
Li, Gang [1 ,2 ,5 ,6 ]
机构：
[1] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci,Fac Med, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Prince Wales Hosp, Fac Med, Lui Che Woo Inst Innovat Med, Shatin, Hong Kong, Peoples R China
[3] Zibo Cent Hosp, Dept Orthopaed, 54 West Gongqingtuan Rd, Zibo, Shandong, Peoples R China
[4] Guangzhou Univ Chinese Med, Clin Med Coll 1, Affiliated Hosp 1, Key Lab Orthopaed & Traumatol, Guangzhou, Guangdong, Peoples R China
[5] Chinese Univ Hong Kong, CUHK ACC Space Med Ctr Hlth Maintenance Musculosk, Shenzhen Res Inst, Shenzhen, Peoples R China
[6] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Inst, Prince Wales Hosp, Room 904,9-F, Shatin, Hong Kong, Peoples R China
来源：
CALCIFIED TISSUE INTERNATIONAL | 2018年 / 103卷 / 02期
基金：
中国国家自然科学基金;
关键词：
MicroRNA-218; Mesenchymal stem cells; Osteogenesis; Femur fracture; TRANSCRIPTION FACTOR; OSTEOBLAST DIFFERENTIATION; ALKALINE-PHOSPHATASE; MINERALIZATION; OSTERIX; RUNX2; EXPRESSION; CIRCUIT; MIR-218;
D O I：
10.1007/s00223-018-0410-8
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
As a regulator of osteogenesis, microRNA-218 (miR-218) is reported to promote osteogenesis of mesenchymal stem cells (MSCs). However, the in vivo osteogenic effect of miR-218 remains elusive. In this study, miR-218 was confirmed to promote osteogenic differentiation of MSCs by stimulating the alkaline phosphatase activity, calcium nodule formation, and osteogenic marker gene expression. For in vivo study, the miR-218-overexpressing BMSCs were locally administrated into the fracture sites in a femur fracture mouse model. Based on the X-rays, micro-computed tomography, mechanical testing, histology, and immunohistochemistry examinations, miR-218 overexpression improved new bone formation and accelerated fracture healing. These findings suggest that miR-218 may be a promising therapeutic target for bone repair in future clinical applications.
引用
收藏
页码：227 / 236
页数：10
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