Optimization of culture medium for anaerobic production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl for microbial enhanced oil recovery

被引:31
|
作者
Zhao, F. [1 ,2 ]
Mandlaa, M. [1 ,2 ]
Hao, J. [3 ]
Liang, X. [1 ,2 ]
Shi, R. [1 ]
Han, S. [1 ]
Zhang, Y. [1 ]
机构
[1] Chinese Acad Sci, Inst Appl Ecol, Key Lab Inst Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China
[2] Univ Chinese Acad Sci, Beijing, Peoples R China
[3] Daqing Oilfield Co Ltd, Oil Prod Factory 2, Daqing, Peoples R China
基金
国家高技术研究发展计划(863计划);
关键词
anaerobic; glycerol; microbial enhanced oil recovery; Pseudomonas stutzeri; responsesurface methodology; rhamnolipid; RESPONSE-SURFACE METHODOLOGY; AERUGINOSA; BIOSURFACTANT; GLYCEROL; CARBON;
D O I
10.1111/lam.12269
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Response surface methodology was employed to enhance the anaerobic production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl. Glycerol is a promising carbon source used to anaerobically produce rhamnolipid. In a Plackett-Burman design, glycerol, KH2PO4 and yeast extract were significant factors. The proposed optimized medium contained the following: 46.55 g l(-1) glycerol; 3 g l(-1) NaNO3; 5.25 g l(-1) K(2)HPO(4 center dot)3H2O; 5.71 g l(-1) KH2PO4; 0.40 g l(-1) MgSO4 center dot 7H(2)O; 0.13 g l(-1) CaCl2; 1.0 g l(-1) KCl; 1.0 g l(-1) NaCl; and 2.69 g l(-1) yeast extract. Using this optimized medium, we obtained an anaerobic yield of rhamnolipid of 3.12 +/- 0.11 g l(-1) with a 0.85-fold increase. Core flooding test results also revealed that Ps. stutzeri Rhl grown in an optimized medium enhanced the oil recovery efficiency by 15.7%, which was 6.6% higher than in the initial medium. Results suggested that the optimized medium is a promising nutrient source that could effectively mobilize oil by enhancing the in situ production of rhamnolipid.
引用
收藏
页码:231 / 237
页数:7
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