Increased quercetinase production by Penicillium olsonii using fractional factorial design

被引：9
|
作者：
Iacazio, G [1 ]
机构：
[1] Fac Sci & Tech St Jerome, UMR CNRS 6117, Lab Bioinorgan Struct, F-13397 Marseille 20, France
来源：
PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 01期
关键词：
quercetinase; enzyme production; fractional factorial design; Penicillium olsonii;
D O I：
10.1016/j.procbio.2004.01.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Medium improvement for quereetinase production by Penicillium olsonii was investigated using a fractional factorial design in two sets of eight experiments with seven variable components each. The tested components were: rutin concentration, nitrogen source and its concentration, KH2PO4, NaCl, MgS04 and yeast extract concentrations, inoculum size, copper and metal salts concentrations and pH value. A final optimisation step, varying only yeast extract concentration, allowed six-fold improvement of the quercetinase activity with respect to initial medium, reaching a maximum activity of 0.708 mumol/min/ml after 9 days of cultivation. (C) 2004 Published by Elsevier Ltd.
引用
收藏
页码：379 / 384
页数：6
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