SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool

被引:31
|
作者
Upadhyay, Santosh Kumar [1 ]
Sharma, Shailesh [1 ]
机构
[1] Govt India, Dept Biotechnol, Natl Agrifood Biotechnol Inst, Ind Area, Mohali 160071, Punjab, India
关键词
RNA; ARABIDOPSIS; MUTAGENESIS; MULTIPLEX;
D O I
10.1155/2014/742482
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.
引用
收藏
页数:4
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