Optical multi-channel interrogation instrument for bacterial colony characterization

被引:2
|
作者
Doh, Iyll-Joon [1 ,2 ]
Kim, Huisung [1 ,2 ]
Sturgis, Jennifer [3 ]
Rajwa, Bartek [4 ]
Robinson, J. Paul [3 ,5 ]
Bae, Euiwon [1 ,2 ]
机构
[1] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Coll Vet Med, W Lafayette, IN 47907 USA
[3] Purdue Univ, Coll Vet Med, Basic Med Sci, W Lafayette, IN 47907 USA
[4] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA
[5] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
来源
PLOS ONE | 2021年 / 16卷 / 02期
关键词
D O I
10.1371/journal.pone.0247721
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A single instrument that includes multiple optical channels was developed to simultaneously measure various optical and associated biophysical characteristics of a bacterial colony. The multi-channel device can provide five distinct optical features without the need to transfer the sample to multiple locations or instruments. The available measurement channels are bright-field light microscopy, 3-D colony-morphology map, 2-D spatial optical-density distribution, spectral forward-scattering pattern, and spectral optical density. The series of multiple morphological interrogations is beneficial in understanding the bio-optical features of a bacterial colony and the correlations among them, resulting in an enhanced power of phenotypic bacterial discrimination. To enable a one-shot interrogation, a confocal laser scanning module was built as an add-on to an upright microscope. Three different-wavelength diode lasers were used for the spectral analysis, and high-speed pin photodiodes and CMOS sensors were utilized as detectors to measure the spectral OD and light-scatter pattern. The proposed instrument and algorithms were evaluated with four bacterial genera, Escherichia coli, Listeria innocua, Salmonella Typhimurium, and Staphylococcus aureus; their resulting data provided a more complete picture of the optical characterization of bacterial colonies.
引用
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页数:16
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