Changing surface properties of artificial lipid membranes at the interface with biopolymer coated gold nanoparticles under normal and redox conditions

被引：3

作者：

Karanth, Sanjai ^{[1
,2
]}

Meesaragandla, Brahmaiah ^{[1
,2
]}

Delcea, Mihaela ^{[1
,2
,3
]}

机构：

[1] Univ Greifswald, Inst Biochem, Felix Hausdorff Str 4, D-17489 Greifswald, Germany

[2] Zentrum Innovat Kompetenz Humorale Immunreakt Kar, ZIK HIKE, Fleischmannstr 42, D-17489 Greifswald, Germany

[3] DZHK DeutschesZentrum Herz Kreislauf Forsch, Partner Site, Greifswald, Germany

来源：

BIOPHYSICAL CHEMISTRY | 2020年 / 267卷

关键词：

Chitosan; Dextran-10; Gold nanoparticles; DMPC; QCM-D; AFM; Nitric oxide; REACTIVE OXYGEN; NITRIC-OXIDE; CELL-MEMBRANE; MOLECULAR-WEIGHT; CHITOSAN; BILAYER; DELIVERY; WATER; DEPOLYMERIZATION; AGGREGATION;

D O I：

10.1016/j.bpc.2020.106465

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gold nanoparticles (NPs) functionalized with biopolymers are increasingly effective in drug-delivery applications. Here, we investigated how chitosan coated NPs and dextran-10 coated NPs regulate their action on DMPC bilayer under normal and stress conditions. We found that chitosan-coated NPs interact with lipid membrane in an intermittent manner, causing lipid loss and partial membrane rupture, while dextran-10 coated NPs mostly induced complete rupture as observed by quartz crystal microbalance. In-situ atomic force microscopy imaging showed that chitosan-treated membranes have a higher surface roughness than those treated with dextran-10. Treatment with 1 mu M nitric oxide (NO) radical caused the release of chitosan ligand from the surface of gold NPs (reduced stability) and its aggregation, but the functionality seemed less influenced. Dextran-10 ligand showed no such behavior, while its action was only delayed. Our findings give insights into possible challenges faced by NPs in-situ and show environment dependent effects of NPs on membranes.

引用

页数：10