Automation of NMR measurements and data evaluation for systematically screening interactions of small molecules with target proteins

被引：45

作者：

Ross, A ^{[1
]}

Schlotterbeck, G ^{[1
]}

Klaus, W ^{[1
]}

Senn, H ^{[1
]}

机构：

[1] Hoffmann LaRoche AG, Dept Biostruct Res PRPCS, CH-4070 Basel, Switzerland

来源：

JOURNAL OF BIOMOLECULAR NMR | 2000年 / 16卷 / 02期

关键词：

flow injection; protein; screening;

D O I：

10.1023/A:1008394910612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this technical note we describe the setup and application of automated sample preparation and usage of flow-through NMR equipment for the characterization of ligand binding on proteins. In addition, we focus on the perspectives of automated analysis of 2D HSQC spectra to identify changes in patterns indicative for ligand binding or changes of sample conditions. In this context we discuss a combination of statistical and non-statistical data analysis.

引用

页码：139 / 146

页数：8

共 50 条

[1] Automation of NMR measurements and data evaluation for systematically screening interactions of small molecules with target proteins
Alfred Ross
Götz Schlotterbeck
Werner Klaus
Hans Senn
Journal of Biomolecular NMR, 2000, 16 : 139 - 146
[2] Automation of measurements and data evaluation in biomolecular NMR screening
Ross, A
Senn, H
DRUG DISCOVERY TODAY, 2001, 6 (11) : 583 - 593
[3] NMR screening and studies of target - ligand interactions
Polshakov, Vladimir I.
Batuev, Evgeniy A.
Mantsyzov, Alexey B.
RUSSIAN CHEMICAL REVIEWS, 2019, 88 (01) : 59 - 98
[4] n®p* Interactions in proteins and small molecules
Raines, Ronald T.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
[5] Reciprocal carbonyl–carbonyl interactions in small molecules and proteins
Abdur Rahim
Pinaki Saha
Kunal Kumar Jha
Nagamani Sukumar
Bani Kanta Sarma
Nature Communications, 8
[6] Microcalorimetric studies of the binding of small molecules to target proteins.
Thomson, JA
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1997, 213 : 154 - MEDI
[7] Target Prediction of Small Molecules with Information of Key Molecular Interactions
Lin, Jung-Hsin
CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2012, 12 (17) : 1903 - 1910
[8] Methods of probing the interactions between small molecules and disordered proteins
Gabriella T. Heller
Francesco A. Aprile
Michele Vendruscolo
Cellular and Molecular Life Sciences, 2017, 74 : 3225 - 3243
[9] Methods of probing the interactions between small molecules and disordered proteins
Heller, Gabriella T.
Aprile, Francesco A.
Vendruscolo, Michele
CELLULAR AND MOLECULAR LIFE SCIENCES, 2017, 74 (17) : 3225 - 3243
[10] Reciprocal carbonyl-carbonyl interactions in small molecules and proteins
Rahim, Abdur
Saha, Pinaki
Jha, Kunal Kumar
Sukumar, Nagamani
Sarma, Bani Kanta
NATURE COMMUNICATIONS, 2017, 8

← 1 2 3 4 5 →