We conducted research to confirm the applicability of a soil CO2 measurement system to estimate changes in the level of soil carbon storage. We monitored the soil surface CO2 efflux (F-c), layer CO2 concentration (C-c), temperature (T-s), and moisture (theta) monthly from September 2018 to September 2020 at 18 locations in a 20-year-old Pinus koraiensis stand in South Korea. The recorded average F-c, C-c, T-s, and. were 519.8 mg C m(-2) h(-1), 1775.7 ppm, 12.1 degrees C, and 13.9%, respectively. The observed F-c and C-c values increased during the growth season (April-October); however, F-c (Q(10) = 2.19) was more sensitive to temperature changes than C-c (Q(10) = 1.74). To investigate the effects of theta on F-c and C-c, they were normalized at Ts = 10 degrees C to F10 and C-10. To perform regression analysis, F-norm and C-norm were calculated by normalizing F-c and C-c to minimize the temperature effects. This. did not explain F-norm and C-norm well as a single independent variable. However, according to the results of multiple nonlinear regression analyses, C-10 and theta explained approximately 70% of F-10 (p < 0.0001). To estimate the precise levels of soil carbon storage, it is essential that various types of forest and climate conditions be monitored.