Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar

Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC). In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated. The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity. A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L). The increment of H2SO4 concentration promoted the yield of xylooligo-saccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis. This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.