Tonsil-derived mesenchymal stem cell-embedded in situ crosslinkable gelatin hydrogel therapy recovers postmenopausal osteoporosis through bone regeneration

被引：23
|
作者：
Kim, Gyungah [1 ,2 ]
Park, Yoon Shin [3 ]
Lee, Yunki [4 ]
Jin, Yoon Mi [1 ,2 ]
Choi, Da Hyeon [3 ]
Ryu, Kyung-Ha [2 ,5 ]
Park, Yoon Jeong [6 ,7 ]
Park, Ki Dong [4 ]
Jo, Inho [1 ,2 ]
机构：
[1] Ewha Womans Univ, Coll Med, Dept Mol Med, Seoul, South Korea
[2] Ewha Womans Univ, Coll Med, Ewha Tonsil Derived Mesenchymal Stem Cells Res Ct, Seoul, South Korea
[3] Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Microbiol, Cheongju, South Korea
[4] Ajou Univ, Dept Mol Sci & Technol, Suwon, South Korea
[5] Ewha Womans Univ, Coll Med, Dept Pediat, Seoul, South Korea
[6] Seoul Natl Univ, Sch Dent, Dent Res Inst, Dept Dent Regenerat Biotechnol, Seoul, South Korea
[7] NIBEC, Cent Res Inst, Seoul, South Korea
来源：
PLOS ONE | 2018年 / 13卷 / 07期
基金：
新加坡国家研究基金会;
关键词：
OSTEOGENIC DIFFERENTIATION; STROMAL CELLS; MOUSE MODEL; TISSUE; VITRO; MARROW; EXPRESSION; DELIVERY; HORMONE; CULTURE;
D O I：
10.1371/journal.pone.0200111
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We investigated therapeutic potential of human tonsil-derived mesenchymal stem cells (TMSC) subcutaneously delivered to ovariectomized (OVX) mice for developing more safe and effective therapy for osteoporosis. TMSC were isolated from tonsil tissues of children undergoing tonsillectomy, and TMSC-embedded in situ crosslinkable gelatin-hydroxyphenyl propionic acid hydrogel (TMSC-GHH) or TMSC alone were delivered subcutaneously to the dorsa of OVX mice. After 3 months, three-dimensionally reconstructed micro-computed tomographic images revealed better recovery of the femoral heads in OVX mice treated with TMSC-GHH. Serum osteocalcin and alkaline phosphatase were also recovered, indicating bone formation only in TMSC-GHH-treated mice, and absence in hypercalcemia or other severe macroscopic deformities showed biocompatibility of TMSC-GHH. Additionally, visceral fat reduction effects by TMSC-GHH further supported their therapeutic potential. TMSC provided therapeutic benefits toward osteoporosis only when embedded in GHH, and showed potential as a supplement or alternative to current therapies.
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页数：17
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