Molecular typing of Francisella tularensis using multiple-locus variable-number tandem-repeat analysis

被引:2
|
作者
Timofeev, V. S. [1 ]
Kudryavtseva, T. Yu. [1 ]
Mokrievich, A. N. [1 ]
Pavlov, V. M. [1 ]
Dyatlov, I. A. [1 ]
机构
[1] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Russia
关键词
tularemia; Francisella tularensis; genotyping; VNTR; MLVA;
D O I
10.3103/S0891416814010066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Variable-number tandem-repeat analysis (VNTR) of 25 loci was used for molecular typing of the Francisella tularensis strains isolated from different regions of Russia and the former Soviet Union. This approach allowed us to subdivide F. tularensis subspecies and determine genotype diversity with regard to geographical prevalence. All 25 loci were examined for their ability to distinguish subspecies and local geographical groups. Forty-two genotypes of the 58 investigated F. tularensis subsp. holarctica isolates were found using cluster VNTR analysis.
引用
收藏
页码:8 / 15
页数:8
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