Evaluation of cobas Influenza A/B & RSV Test for Diagnosis of Equine Influenza

被引：1

作者：

Nemoto, Manabu ^{[1
]}

Okita, Noriyuki ^{[2
]}

Kitahata, Masayoshi ^{[2
]}

Bannai, Hiroshi ^{[1
]}

Tsujimura, Koji ^{[1
]}

Kinoshita, Yuta ^{[1
]}

Kambayashi, Yoshinori ^{[1
]}

Cullinane, Ann ^{[3
]}

Yamanaka, Takashi ^{[1
]}

Ohta, Minoru ^{[1
]}

机构：

[1] Japan Racing Assoc, Equine Res Inst, Shimotsuke, Tochigi, Japan

[2] Roche Diagnost KK, Mol Diagnost, Tokyo, Japan

[3] Irish Equine Ctr, Virol Unit, Johnstown, Kildare, Ireland

来源：

JOURNAL OF EQUINE VETERINARY SCIENCE | 2020年 / 94卷

关键词：

cobas liat system; Diagnosis; Equine influenza virus; Real-time reverse transcription polymerase chain reaction; VIRUSES;

D O I：

10.1016/j.jevs.2020.103249

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A rapid and sensitive diagnostic method is needed to help prevent the spread of equine influenza virus. The cobas Influenza A/B & RSV test for the cobas Liat system (Roche Diagnostics) is based on real-time reverse transcription polymerase chain reaction and is designed to broadly detect influenza A virus RNA within 20 minutes. It detected a broad range of equine influenza virus strains, and detected equine influenza virus RNA from nasal swabs of infected horses at the same level as real-time reverse transcription polymerase chain reaction, although it returned some invalid results (7.7%). This suggests that cobas Influenza A/B & RSV test is highly sensitive to equine influenza virus, but should be improved to reduce the rate of invalid results. (C) 2020 Elsevier Inc. All rights reserved.

引用

页数：4

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