Tissue-specific molecular heterogeneity of human growth hormone-releasing hormone receptor protein

被引：13
|
作者：
Fujinaka, Y
Yokogoshi, Y
Zhang, CY
Okura, T
Kitagawa, K
Saito, S
机构：
[1] UNIV TOKUSHIMA,SCH MED,DEPT INTERNAL MED 1,TOKUSHIMA 770,JAPAN
[2] NIIGATA COLL PHARM,DEPT CHEM & PHARMACEUT TECHNOL,NIIGATA 95021,JAPAN
来源：
FEBS LETTERS | 1996年 / 394卷 / 01期
关键词：
GHRH; GHRH receptor; anti-peptide antibody; molecular heterogeneity; immunoblotting; kidney;
D O I：
10.1016/0014-5793(96)00917-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A site-directed anti-peptide antibody (anti-hGHRHRc18) was generated against the cytoplasmic tail of human GHRH receptor. The dissociation constant (K-d) and the antibody binding site (AbT) of anti-hGHRHRc18 were 2.5 nmol/l and 0.54 nmol/l, respectively, In an immunoblotting experiment, affinity-purified anti-hGHRHRc18 specifically recognized a single 50-kDa protein in human pituitary, In a screening of the expression of GHRH receptor protein in extra-pituitary tissues, only human kidney showed a single 52-kDa protein, Our results suggest that the GHRH receptor protein exhibits tissue-specific molecular heterogeneity.
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页码：1 / 4
页数：4
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