Autoantibody panel on small extracellular vesicles for the early detection of lung cancer

被引:5
|
作者
Hua, Yan [1 ,2 ]
Dai, Chunyang [1 ,2 ]
He, Qing [3 ]
Cai, Xueqin [1 ,2 ]
Li, Ming [1 ,2 ]
机构
[1] Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Lab Diagnost, Div Life Sci & Med, Hefei 230031, Peoples R China
[2] Core Unit Natl Clin Res Ctr Lab Med, Hefei, Peoples R China
[3] Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei 230031, Peoples R China
关键词
Autoantibody; Small extracellular vesicles; Biomarker; Lung cancer; Immune escape; IMMUNOTHERAPY; ANTIBODY; EXOSOMES;
D O I
10.1016/j.clim.2022.109175
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This study evaluated a different form of autoantibody, which is on small extracellular vesicles (sEVs), as a biomarker for early detection of lung cancer. The sEVs were isolated from plasma by ultracentrifugation and validated with morphology and typical markers. The autoantibody levels were quantified by enzyme-linked immunosorbent assay, and further analysis indicated that the autoantibody panel on sEVs was better than that from serum to distinguish benign lung disease (n = 32) from lung cancer (n = 90). In the prospective study, autoantibody on sEVs performed better in identification of patients with a higher risk of lung cancer. Further-more, with immunogold labeling transmission electron microscopy, Nanoflow cytometry and binding tests, we illustrated that the autoantibodies could bind to the antigens on sEVs, which may explain the detected auto -antibodies on sEVs. Besides, the binding resulted in the attenuation of complement-mediated cytotoxicity, which may contribute to the immune escape of lung cancer.
引用
收藏
页数:10
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