Molecular and biological characterization of natural variants of Citrus dwarfing viroid

被引：9

作者：

Murcia, N. ^{[1
]}

Bernad, L. ^{[1
]}

Serra, P. ^{[1
]}

Bani Hashemian, S. M. ^{[1
]}

Duran-Vila, Nuria ^{[1
]}

机构：

[1] Ctr Protecc Vegetal & Biotecnol, Inst Valenciano Invest Agr, Dept Protecc Vegetal & Biotecnol, Valencia 46113, Spain

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 08期

关键词：

SEQUENCE;

D O I：

10.1007/s00705-009-0430-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Citrus dwarfing viroid has been proposed as an agent to control tree size in high-density plantations. Thirty-three field isolates have been characterized, and the most frequent sequence/s have been identified. Five distinct variants were selected for biological characterization. Symptom expression analysis demonstrated a good correlation between leaf/stem symptoms and plant growth. The discriminating nucleotide sequence differences included two deletions and an insertion resulting in a reorganization of the base pairing of the terminal left loop, two (G42 -> A and C52 -> U) changes found in one of the variants, and as many as thirteen changes located in the right and left regions flanking the CCR.

引用

页码：1329 / 1334

页数：6

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