HaloTag protein-mediated site-specific conjugation of bioluminescent proteins to quantum dots

被引:120
|
作者
Zhang, Yan
So, Min-kyung
Loening, Andreas M.
Yao, Hequan
Gambhir, Sanjiv S.
Rao, Jianghong
机构
[1] Stanford Univ, Sch Med, Dept Radiol, Mol Imaging Program, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
关键词
bioluminescence resonance energy transfer; conjugation; luciferase; protein structures; quantum dots;
D O I
10.1002/anie.200601197
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
On the dot: A genetically engineered haloalkane dehalogenase was used to conjugate Renilla luciferase to quantum dots (see picture). The quantum dots can emit light through bioluminescence resonance energy transfer (BRET). This specific conjugation occurs upon simple mixing under mild conditions, and may be applied for specific in vivo labeling of proteins with quantum dots for imaging. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:4936 / 4940
页数:5
相关论文
共 50 条
  • [1] HaloTag protein-mediated specific labeling of living cells with quantum dots
    So, Min-Kyung
    Yao, Hequan
    Rao, Jianghong
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 374 (03) : 419 - 423
  • [2] Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
    Charalambous A.
    Andreou M.
    Skourides P.A.
    [J]. Journal of Nanobiotechnology, 7 (1)
  • [3] Site-Specific Conjugation of Polymers to Proteins
    Wang, Yanjing
    Wu, Chi
    [J]. BIOMACROMOLECULES, 2018, 19 (06) : 1804 - 1825
  • [4] Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
    Charalambous, Anna
    Antoniades, Ioanna
    Christodoulou, Neophytos
    Skourides, Paris A.
    [J]. JOURNAL OF NANOBIOTECHNOLOGY, 2011, 9
  • [5] Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
    Anna Charalambous
    Ioanna Antoniades
    Neophytos Christodoulou
    Paris A Skourides
    [J]. Journal of Nanobiotechnology, 9
  • [6] Site-specific folate conjugation to a cytotoxic protein
    Smith, Bryan D.
    Higgin, Joshua J.
    Raines, Ronald T.
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2011, 21 (17) : 5029 - 5032
  • [7] Site-specific conjugation of RAFT polymers to proteins via expressed protein ligation
    Xia, Yan
    Tang, Shengchang
    Olsen, Bradley D.
    [J]. CHEMICAL COMMUNICATIONS, 2013, 49 (25) : 2566 - 2568
  • [8] Nonnatural Amino Acids for Site-Specific Protein Conjugation
    de Graaf, Albert J.
    Kooijman, Marlous
    Hennink, Wim E.
    Mastrobattista, Enrico
    [J]. BIOCONJUGATE CHEMISTRY, 2009, 20 (07) : 1281 - 1295
  • [9] Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots
    Sunbul, Murat
    Yen, Michelle
    Zou, Yekui
    Yin, Jun
    [J]. CHEMICAL COMMUNICATIONS, 2008, (45) : 5927 - 5929
  • [10] A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins
    Wang, Yan
    Dong, Mingming
    Ye, Mingliang
    [J]. ELECTROPHORESIS, 2019, 40 (16-17) : 2125 - 2128