HaloTag protein-mediated site-specific conjugation of bioluminescent proteins to quantum dots

被引：120
|
作者：
Zhang, Yan
So, Min-kyung
Loening, Andreas M.
Yao, Hequan
Gambhir, Sanjiv S.
Rao, Jianghong
机构：
[1] Stanford Univ, Sch Med, Dept Radiol, Mol Imaging Program, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 30期
关键词：
bioluminescence resonance energy transfer; conjugation; luciferase; protein structures; quantum dots;
D O I：
10.1002/anie.200601197
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
On the dot: A genetically engineered haloalkane dehalogenase was used to conjugate Renilla luciferase to quantum dots (see picture). The quantum dots can emit light through bioluminescence resonance energy transfer (BRET). This specific conjugation occurs upon simple mixing under mild conditions, and may be applied for specific in vivo labeling of proteins with quantum dots for imaging. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：4936 / 4940
页数：5
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