Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins

被引：408

作者：

Lee, In Su

Lee, Nohyun

Park, Jongnam

Kim, Byung Hyo

Yi, Yong-Weon

Kim, Taeuk

Kim, Tae Kook

Lee, In Hwan

Paik, Seung R.

Hyeon, Taeghwan ^{[1
]}

机构：

[1] Seoul Natl Univ, Natl Creat Res Initiat Ctr Oxide Nanocrystalline, Seoul 151744, South Korea

[2] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151744, South Korea

[3] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 33期

关键词：

D O I：

10.1021/ja063177n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads. Copyright © 2006 American Chemical Society.

引用

页码：10658 / 10659

页数：2

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