Angiopoietin-1 and vascular endothelial growth factor induce expression of inflammatory cytokines before angiogenesis

被引:61
|
作者
Aplin, Alfred C.
Gelati, Maurizio
Fogel, Eric
Carnevale, Edvige
Nicosia, Roberto F.
机构
[1] VA Puget Sound Hlth Care Syst, Div Pathol & Lab Med, Lab S113, Seattle, WA 98108 USA
[2] Carlo Besta Inst, Lab Neurobiol & Neuroregenerat Med, Milan, Italy
[3] Univ Washington, Dept Pathol, Seattle, WA 98195 USA
关键词
aorta; oligonucleotide microarray; gene profiling;
D O I
10.1152/physiolgenomics.00048.2006
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The purpose of this study was to identify novel transcriptional events occurring in the aortic wall before angiogenesis. We used a defined tissue culture system that takes advantage of the capacity of rat aortic rings to generate neovessels ex vivo in response to angiogenic factor stimulation. Total RNA isolated from aortic rings 18 h posttreatment with angiopoietin (Ang)-1 or vascular endothelial growth factor (VEGF) was used to probe oligonucleotide microarrays. Many genes were up- or downregulated by either Ang-1 or VEGF, with a subset being affected by treatment with both growth factors. Grouping of genes by biological function revealed that Ang-1 and VEGF both upregulated a host of immune-related genes including many inflammatory cytokines. A mixture of the Ang-1- and VEGF-induced cytokines stimulated the spontaneous angiogenic response of aortic rings and was synergistic with a low dose of recombinant VEGF. This effect was associated with enhanced recruitment of adventitial macrophages and dendritic cells in the angiogenic outgrowths. Thus Ang-1 and VEGF activate the innate immune system of the vessel wall, stimulating the production of proangiogenic inflammatory cytokines before the emergence of neovessels. This hitherto unreported feature of the angiogenic response might represent an important early component of the cellular and molecular cascade responsible for the angiogenic response of the aortic wall.
引用
收藏
页码:20 / 28
页数:9
相关论文
共 50 条
  • [1] Angiopoietin-1 and vascular endothelial growth factor expression in human esophageal cancer
    Nagata, J
    Kijima, H
    Hatanaka, H
    Tokunaga, T
    Kamochi, J
    Abe, Y
    Takagi, A
    Mine, T
    Yamazaki, H
    Nakamura, M
    Ueyama, Y
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2002, 10 (04) : 423 - 426
  • [2] Effects of angiopoietin-1 on vascular endothelial growth factor-induced angiogenesis in the mouse brain
    Zhu, Y.
    Shwe, Y.
    Du, R.
    Chen, Y.
    Shen, F. X.
    Young, W. L.
    Yang, G. Y.
    [J]. BRAIN EDEMA XIII, 2006, 96 : 438 - +
  • [3] Angiogenesis in cholangiocellular carcinoma: Expression of vascular endothelial growth factor, angiopoietin-1/2, thrombospondin-1 and clinicopathological significance
    Di, T
    Nagano, H
    Yamamoto, H
    Wada, H
    Nakamura, M
    Kondo, M
    Ota, H
    Yoshioka, S
    Kato, H
    Damdinsuren, B
    Marubashi, S
    Miyamoto, A
    Takeda, Y
    Umeshita, K
    Dono, K
    Wakasa, K
    Monden, M
    [J]. ONCOLOGY REPORTS, 2006, 15 (03) : 525 - 532
  • [4] Plasma vascular endothelial growth factor, angiopoietin-1 and angiopoietin-2 in diabetes
    Severinoy, Perevod A. S.
    [J]. DIABETES MELLITUS, 2005, 8 (04): : 62 - 63
  • [5] Aspirin reduces platelet vascular endothelial growth factor and angiopoietin-1
    Nadar, SK
    Blann, AD
    Lip, GYH
    [J]. AMERICAN JOURNAL OF HYPERTENSION, 2004, 17 (05) : 7A - 8A
  • [6] Coadministration of angiopoietin-1 and vascular endothelial growth factor enhances collateral vascularization
    Chae, JK
    Kim, I
    Lim, ST
    Chung, MJ
    Kim, WH
    Kim, HG
    Ko, JK
    Koh, GY
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2000, 20 (12) : 2573 - 2578
  • [7] Immunomodulatory role of vascular endothelial growth factor and angiopoietin-1 in airway remodeling
    Makinde, Toluwalope
    Murphy, Richard F.
    Agrawal, Devendra K.
    [J]. CURRENT MOLECULAR MEDICINE, 2006, 6 (08) : 831 - 841
  • [8] Vascular endothelial growth factor and angiopoietin-1 expression throughout acetaminophen-induced liver toxicity and regeneration
    Papastefanou, V. P.
    Bozas, E.
    Nicolopoulou-Stamati, P.
    Grypioti, A. D.
    Garyfallidis, S.
    Moutsatsou, P.
    Mykoniatis, M. G.
    [J]. JOURNAL OF HEPATOLOGY, 2007, 46 : S144 - S144
  • [9] Combined gene transfer of angiopoietin-1 and vascular endothelial growth factor synergistically induced angiogenesis in swine myocardial ischemia.
    Zu, L
    Jiang, J
    Chen, M
    Yang, Y
    Guo, Y
    Chen, L
    Zhou, A
    Gao, W
    [J]. AMERICAN JOURNAL OF CARDIOLOGY, 2004, 94 (6A): : 188E - 189E
  • [10] Adrenomedullin and Angiopoietin-1 Additively Restore Erectile Function in Diabetic Rats: Comparison with the Combination Therapy of Vascular Endothelial Growth Factor and Angiopoietin-1
    Nishimatsu, Hiroaki
    Suzuki, Etsu
    Nomiya, Akira
    Niimi, Aya
    Suzuki, Motofumi
    Fujimura, Tetsuya
    Fukuhara, Hiroshi
    Homma, Yukio
    [J]. JOURNAL OF SEXUAL MEDICINE, 2013, 10 (07): : 1707 - 1719