HYDROGEL MICROARRAY FOR LOW-COST SNP SCREENING

被引：0
|
作者：
Wang Hui [1 ,2 ]
Chen Zhiyao [1 ,3 ]
Li Youzhi [1 ]
Huang Huan [1 ]
Zhou Guohua [1 ,2 ,3 ]
机构：
[1] Huadong Res Inst Med & Biomech, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing 210002, Jiangsu, Peoples R China
[3] Nanjing Univ, Sch Med, Nanjing 210002, Jiangsu, Peoples R China
来源：
PROGRESS ON POST-GENOME TECHNOLOGIES AND MODERN NATURAL PRODUCTS, 2011 | 2011年
关键词：
Hydrogel chip; single nucleotide polymorphisms; specific primer extension;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This paper propoed a hydrogel chip platform to established a low-cost method for screening of SNP. The proposed method based on copolymerization of acrylamideamidemodified PCR products with acrylamideamide monomers and acrylamidemodified slides to prepare gelbased microarray. Then the array was hybridized with two types of hybridization probes for SNP respectively. Electrophoresis was used to remove the nonspecifically bound targets and mismatches. After that, the array was extended with dNTPs containing Cy5-dCTP. Finally, genotyping was based on fluorescence scanning. As the using of general probe during the hybridization, the specific labeled probesis non-essential for each SNPwhich can reduce the cost of screening. 16 samples of OLR1-C14417G were detected, the results are consistent with Sequencing. The established hydrogel chip platform is a low-cost method for detection of SNP.
引用
收藏
页码：313 / 315
页数：3
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