2-methyladenosine-substituted 2′,5′-oligoadenylates:: Conformations, 2-5A binding and catalytic activities with human ribonuclease L

被引：6

作者：

Kitade, Y ^{[1
]}

Wakana, M

Tsuboi, T

Yatome, C

Bayly, SF

Player, MR

Torrence, PF

机构：

[1] Gifu Univ, Fac Engn, Dept Biomol Sci, Lab Mol Biochem, Gifu 5011193, Japan

[2] NIDDKD, Sect Biomed Chem, Med Chem Lab, NIH, Bethesda, MD 20892 USA

[3] No Arizona Univ, Dept Chem, Flagstaff, AZ 86011 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0960-894X(99)00703-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

2-Methyladenosine-substituted analogues of 2-5A, p5'A2'p5'A2'p5'(me(2)A), p5'(me(2)A)2'p5'A2'p5'A, and p5'(me(2)A) 2'p5'(me(2)A)2'p5'(me(2)A), were prepared via a modification of a lead ion-catalyzed ligation reaction. These 5'-monophosphates were subsequently converted into the corresponding 5'-triphosphates. Both binding and activation of human recombinant RNase L by various 2-methyladenosine-substituted 2-5A analogues were examined. Among the 2-5A analogues, p5'A2'p5'A2'p5'(me(2)A) showed the strongest binding affinity and was as effective as 2-5A itself as an activator of RNase L. The CD spectra of both p5'(me(2)A)2'p5'A2'p5'A and p5'A2'p5'A2'p5'(me(2)A) were superimposable on that of p5'A2'p5'A2'p5'A. indicative of an anti orientation about the base-glycoside bonds as in naturally occurring 2-5A. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：329 / 331

页数：3

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