Thermodynamics and kinetics of lysozyme adsorption onto two kinds of weak cation exchangers

被引：11

作者：

Cheng, Yong-Mei ^{[1
]}

Jin, Xiong-Hua ^{[2
,4
]}

Gao, Dong ^{[3
]}

Xia, Hai-Feng ^{[2
]}

Chen, Jing-Hua ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China

[2] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China

[3] Northwest Univ, Shaanxi Key Lab Modern Separat Sci, Inst Modern Separat Sci, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710068, Peoples R China

[4] Zhejiang Hisun Pharmaceut Co Ltd, Taizhou 318000, Peoples R China

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2013年 / 18卷 / 05期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

ion exchange; lysozyme; agarose beads; acrylic acid based resin; thermodynamics and kinetics; HYDROPHOBIC-INTERACTION CHROMATOGRAPHY; PURIFICATION; PROTEINS; MEMBRANE; ULTRAFILTRATION; RETENTION;

D O I：

10.1007/s12257-012-0765-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present work investigated the adsorption behaviors of lysozyme onto weak cation exchangers at different temperatures. The adsorption isotherm, adsorption thermodynamics and adsorption kinetics were studied. The results indicate that the adsorption of lysozyme onto acrylic acid copolymer based beads (Hydrolite D115) is spontaneous and exothermic, while that onto agarose based beads (CM Sepharose 6 Fast Flow) is also spontaneous, but endothermic. The pseudo second-order kinetic model fits well to the dynamic adsorption experimental data, and the kinetic results are also in concert with the adsorption thermodynamics.

引用

页码：950 / 955

页数：6

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