Comparison of the Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa ProFlu plus ™ assay for detecting influenza and respiratory syncytial viruses

被引：10
|
作者：
Selvaraju, Suresh B. [1 ]
Bambach, Adrienne V. [1 ]
Leber, Amy L. [2 ]
Patru, Maria-Magdalena [1 ]
Patel, Anami [3 ]
Menegus, Marilyn A. [1 ]
机构：
[1] Univ Rochester, Med Ctr, Rochester, NY 14642 USA
[2] Nationwide Childrens Hosp, Columbus, OH 43205 USA
[3] LeBonheur Childrens Hosp, Memphis, TN 38103 USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 80卷 / 01期
关键词：
Simplexa; FOCUS nucleic acid extraction-dependent assay; Flu and RSV detection; Prodessa Proflu assay; PCR ASSAYS; 2009; H1N1; IDENTIFICATION; INFECTION;
D O I：
10.1016/j.diagmicrobio.2013.11.015
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The relative performance of 2 widely used reverse transcription polymerase chain reaction (RT-PCR) assays, the Focus diagnostics Simplexa(TM) Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa Proflu+(TM) assay, was evaluated using 735 prospectively and retrospectively collected nasopharyngeal swab specimens. Overall, the assays showed positive and negative agreements of 100% and 99.7% for influenza A, 98.1% and 99.9% for influenza B, and 99.3% and 99.5% for respiratory syncytial virus. The relative analytical sensitivity of the 2 assays was also similar. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：50 / 52
页数：3
相关论文
共 17 条
  • [1] Comparative Evaluation of the Nanosphere Verigene RV plus Assay and the Simplexa Flu A/B & RSV Kit for Detection of Influenza and Respiratory Syncytial Viruses
    Alby, Kevin
    Popowitch, Elena B.
    Miller, Melissa B.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (01) : 352 - 353
  • [2] Comparison of the Focus Diagnostics Simplexa Flu A/B & RSV Direct Assay with the Prodesse ProFlu plus Assay for Detection of Influenza A Virus (IAV), Influenza B Virus (IBV), and Respiratory Syncytial Virus (RSV) in Clinical Specimens
    Sutton, B. C.
    Maggert, K.
    Rowell, B.
    Etter, R.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2014, 16 (06): : 727 - 728
  • [3] Prospective Comparison of ProFlu plus TM with Simplexa Flu A/B & RSV, Eragen ASR and LightCycler PCR for the Detection of Influenza A/B and RSV in Respiratory Specimens
    Espy, M.
    Sloan, L. M.
    Schneider, S. K.
    Pritt, B. S.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2012, 14 (06): : 672 - 673
  • [4] Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu plus RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu
    Cohen, Daniel M.
    Kline, Jennifer
    May, Larissa S.
    Harnett, Glenn Eric
    Gibson, Jane
    Liang, Stephen Y.
    Rafique, Zubaid
    Rodriguez, Carina A.
    McGann, Kevin M.
    Gaydos, Charlotte A.
    Mayne, Donna
    Phillips, David
    Cohenl, Jason
    JOURNAL OF CLINICAL MICROBIOLOGY, 2018, 56 (02)
  • [5] Evaluation of Simplexa Flu A/B & RSV for Direct Detection of Influenza Viruses (A and B) and Respiratory Syncytial Virus in Patient Clinical Samples
    Hindiyeh, Musa
    Kolet, Liat
    Meningher, Tal
    Weil, Merav
    Mendelson, Ella
    Mandelboim, Michal
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (07) : 2421 - 2424
  • [6] Evaluation of focus diagnostics KINEDx Flu/RSV real-time PCR assay versus prodesse ProFlu-1 real-time PCR assay for the detection of Respiratory Syncytial Virus A/B, influenza A and B viruses
    Chen, J.
    Ramos, A.
    Mai, H.
    Chen, F.
    Eleasar, E.
    Watt, P. M.
    Tabb, M. M.
    Lu, N.
    Aye, M.
    An, S.
    Dempsey, S.
    Cheng, C. S.
    Hogrefe, W.
    Lee, M.
    Kong, L.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2007, 9 (05): : 677 - 677
  • [7] Accurate PCR Detection of Influenza A/B and RSV Using the Cepheid Xpert Flu plus RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu
    Gibson, J. S.
    Rodriguez, C.
    Bruce, E.
    McGann, K.
    Gunsolue, B.
    May, L.
    Harnett, G.
    Mayer, M.
    Gaydos, C.
    Barnes, M.
    Cohen, D.
    Kline, J.
    Liang, S.
    House, S.
    Rafique, Z.
    Cohen, J.
    Robak, N.
    Phillips, D.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2016, 18 (06): : 980 - +
  • [8] Influenza and respiratory syncytial virus detection in clinical specimens without nucleic acid extraction using FOCUS direct disc assay is substantially equivalent to the traditional methods and the FOCUS nucleic acid extraction-dependent RT-PCR assay
    Selvaraju, Suresh B.
    Tierney, David
    Leber, Amy L.
    Patel, Anami
    Earley, Amalia K.
    Jaiswal, Dipeshkumar
    Menegus, Marilyn A.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 78 (03) : 232 - 236
  • [9] Clinical performance of the Xpert® Xpress Flu/RSV assay for the detection of influenza A, B, and respiratory syncytial virus on ESwab™ medium
    Gast, Karin B.
    Vrolijk, Angela C. I. M.
    Bergmans, Anneke M. C.
    Geelen, Tanja H.
    Kluytmans, Jan A. J. W.
    Pas, Suzan D.
    JOURNAL OF CLINICAL VIROLOGY PLUS, 2022, 2 (01):
  • [10] Prospective and retrospective evaluation of the Cepheid Xpert® Flu/RSV XC assay for rapid detection of influenza A, influenza B, and respiratory syncytial virus
    Salez, Nicolas
    Nougairede, Antoine
    Ninove, Laetitia
    Zandotti, Christine
    de Lamballerie, Xavier
    Charrel, Remi N.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2015, 81 (04) : 256 - 258
12