On-the-fly fluorescence lifetime determination with total emission detection in HPLC

被引：17
|
作者：
Dvorak, MA [1 ]
Oswald, GA [1 ]
VanBenthem, MH [1 ]
Gillispie, GD [1 ]
机构：
[1] N DAKOTA STATE UNIV,DEPT CHEM,FARGO,ND 58105
来源：
ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 17期
关键词：
D O I：
10.1021/ac961095j
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The entire fluorescence decay profile during HPLC elution has been directly measured on-the-fly in HPLC at higher sensitivity than in previous literature reports, The fluorescence is excited with the fourth harmonic (266 mn) of a pulsed Nd:YAG laser system and detected broadband with a photomultiplier tube and a digital storage oscilloscope. Detection limits in the range 1-10 ppb are found for several individual polycyclic aromatic hydrocarbons (PAHs) when the total time-integrated fluorescence is analysed. The chromatograms of PAH mixtures containing 8-10 species were lifetime analysed with a simple phase plane analysis, in which a single lifetime is determined from the fluorescence decay profile for each point on the chromatogram. The determination of lifetimes under coelution conditions is also illustrated and discussed.
引用
收藏
页码：3458 / 3464
页数：7
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