A highly selective direct method of detecting sulphate-reducing bacteria in crude oil

被引：20
|
作者：
Tanaka, Y [1 ]
Sogabe, M [1 ]
Okumura, K [1 ]
Kurane, R [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Res Inst Biol Resources, Cent 6, Tsukuba, Ibaraki 3058566, Japan
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2002年 / 35卷 / 03期
关键词：
D O I：
10.1046/j.1472-765X.2002.01175.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: The aim of this work was to develop a highly selective method of detecting sulphate-reducing bacteria (SRB) in crude oil. Methods: A pair of PCR primers was designed based on an alignment of the nucleotide sequence of the 16S rRNA genes from the Desulfovibrionaceae family. DNA extraction from crude oil was performed by the method using zirconia beads and a stool kit. Results: The PCR specifically detected Desulfovibrio and Desulfomicrobium in a sediment sample. When nucleic acids extracted directly from crude oil were used for the PCR, 16SrRNAgenes of Desulfovibrio and Thermodesulforhabdus norvegicus were detected. Impact of study: A simple direct method for detection of the SRB in crude oil using PCR was established.
引用
收藏
页码：242 / 246
页数：5
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