Somatic cell nuclear transfer in zebrafish

被引:0
|
作者
Siripattarapravat, Kannika [2 ]
Pinmee, Boonya [1 ]
Venta, Patrick J. [3 ,4 ]
Chang, Chia-Cheng [5 ]
Cibelli, Jose B. [1 ,6 ,7 ]
机构
[1] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA
[2] Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA
[3] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
[4] Michigan State Univ, Dept Small Anim Clin Sci, E Lansing, MI 48824 USA
[5] Michigan State Univ, Dept Pediat Human Dev, E Lansing, MI 48824 USA
[6] Michigan State Univ, Dept Physiol, E Lansing, MI 48824 USA
[7] Programa Andaluz Terapia Celular & Med Regenerat, Andalucia, Spain
关键词
D O I
10.1038/NMETH.1369
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
引用
收藏
页码:733 / 736
页数:4
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