Regulation of Glucose Transporter Expression in Human Intestinal Caco-2 Cells following Exposure to an Anthocyanin-Rich Berry Extract

被引:100
|
作者
Alzaid, Fawaz [1 ]
Cheung, Hoi-Man [1 ]
Preedy, Victor R. [1 ]
Sharp, Paul A. [1 ]
机构
[1] Kings Coll London, Diabet & Nutr Sci Div, London WC2R 2LS, England
来源
PLOS ONE | 2013年 / 8卷 / 11期
关键词
RAT SMALL-INTESTINE; SUGAR ABSORPTION; DIFFERENTIAL EXPRESSION; DIFFUSIVE COMPONENT; DIETARY POLYPHENOLS; EPITHELIAL-CELLS; APICAL GLUT2; SGLT1; MICE; ENTEROCYTES;
D O I
10.1371/journal.pone.0078932
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Polyphenols contained within plant tissues are consumed in significant amounts in the human diet and are known to influence a number of biological processes. This study investigated the effects of an anthocyanin-rich berry-extract on glucose uptake by human intestinal Caco-2 cells. Acute exposure (15 min) to berry extract (0.125%, w/v) significantly decreased both sodium-dependent (Total uptake) and sodium-independent (facilitated uptake) H-3-D-glucose uptake. In longer-term studies, SGLT1 mRNA and GLUT2 mRNA expression were reduced significantly. Polyphenols are known to interact directly with glucose transporters to regulate the rate of glucose absorption. Our in vitro data support this mechanism and also suggest that berry flavonoids may modulate post-prandial glycaemia by decreasing glucose transporter expression. Further studies are warranted to investigate the longer term effects of berry flavonoids on the management of glycaemia in human volunteers.
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页数:6
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