Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

被引：116

作者：

Taura, Daisuke ^{[1
]}

Noguchi, Michio ^{[1
]}

Sone, Masakatsu ^{[1
]}

Hosoda, Kiminori ^{[1
]}

Mori, Eisaku ^{[1
]}

Okada, Yohei ^{[2
]}

Takahashi, Kazutoshi ^{[3
,4
]}

Homma, Koichiro ^{[1
,5
]}

Oyamada, Naofumi ^{[1
]}

Inuzuka, Megumi ^{[1
]}

Sonoyama, Takuhiro ^{[1
]}

Ebihara, Ken ^{[1
]}

Tamura, Naohisa ^{[1
]}

Itoh, Hiroshi ^{[5
]}

Suemori, Hirofumi ^{[6
]}

Nakatsuji, Norio ^{[7
,8
]}

Okano, Hideyuki ^{[2
]}

Yamanaka, Shinya ^{[3
,4
]}

Nakao, Kazuwa ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Sakyo Ku, Kyoto 6068507, Japan

[2] Keio Univ, Sch Med, Dept Physiol, Tokyo 160, Japan

[3] Kyoto Univ, Inst Frontier Med Sci, Dept Stem Cell Biol, Kyoto 6068507, Japan

[4] Inst Integrated Cell Mat Sci, Ctr iPS Cell Res & Applicat CiRA, Kyoto, Japan

[5] Keio Univ, Sch Med, Dept Internal Med, Tokyo, Japan

[6] Kyoto Univ, Inst Frontier Med Sci, Stem Cell Res Ctr, Lab Embryon Stem Cell Res, Kyoto 6068507, Japan

[7] Kyoto Univ, Inst Frontier Med Sci, Dept Dev & Differentiat, Kyoto 6068507, Japan

[8] Kyoto Univ, Inst Integrated Cell Mat Sci iCeMS, Kyoto 6068507, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 06期

关键词：

Adipogenesis; Adipocyte; Stem cell; Differentiation; LEPTIN-REPLACEMENT THERAPY; MOUSE; ADIPOCYTES; DERIVATION; PRIMATE;

D O I：

10.1016/j.febslet.2009.02.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-L-ornithine and fibronectin-coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBP alpha, PPAR gamma 2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1029 / 1033

页数：5

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