A Desaturase Gene Involved in the Formation of 1,14-Nonadecadiene in Synechococcus sp Strain PCC 7002

被引：13
|
作者：
Mendez-Perez, Daniel [1 ]
Herman, Nicolaus A. [1 ]
Pfleger, Brian F. [1 ,2 ]
机构：
[1] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA
[2] Univ Wisconsin, Microbiol Doctoral Training Program, Madison, WI USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2014年 / 80卷 / 19期
关键词：
ACYL-LIPID DESATURASES; BLUE-GREEN-ALGAE; PROTEIN; BIOSYNTHESIS;
D O I：
10.1128/AEM.01615-14
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The marine cyanobacterium Synechococcus sp. strain PCC 7002 synthesizes two alkenes, 1-nonadecene and 1,14-nonadecadiene. Whereas the genetic basis for the biosynthesis of the terminal double bond in both alkenes has been characterized, the origin of the internal double bond in 1,14-nonadecadiene has not. In this study, we demonstrate that a gene encoding an uncharacterized desaturase is involved in the formation of the internal double bond of 1,14-nonadecadiene. Further, at low temperatures, the desaturase gene is essential for growth, and in wild-type cells the levels of 1,14-nonadecadiene increase relative to that of cells grown at 38 degrees C. These data suggest that 1,14-nonadecadiene plays a role in responding to cold stress.
引用
收藏
页码：6073 / 6079
页数：7
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