Mechanism of enhancement of prochymosin renaturation by solubilization of inclusion bodies at alkaline pH

被引：5
|
作者：
Zhang, ZZ [1 ]
Zhang, YY [1 ]
Yang, KY [1 ]
机构：
[1] CHINESE ACAD SCI, INST MICROBIOL, BEIJING 100080, PEOPLES R CHINA
来源：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 1997年 / 40卷 / 02期
关键词：
solubilization of inclusion bodies; renaturation of recombinant prochymosin;
D O I：
10.1007/BF02882045
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The renaturation efficiency of recombinant prochymosin depends on not only the renaturation conditions but also the solubilization (denaturation) conditions. Compared with pH 8, solubilization of prochymosin-containing inclusion bodies at pH 11 (8 mol/L urea) results in onefold increase of renaturation efficiency (similar to 40% vs. similar to 20%). Alkaline pH facilitates the solubilization of inclusion bodies via the breakage of intermolecular disulfide bonds. Moreover, alkaline pH renders prochymosin molecules to be in a more reduced and more unfolded state which undergoes refolding readily.
引用
收藏
页码：169 / 175
页数：7
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