Evaluation of Babesia bigemina 200 kDa recombinant antigen in enzyme-linked immunosorbent assay

被引：6
|
作者：
Altangerel, Khukhuu [1 ]
Alhassan, Andy [1 ]
Iseki, Hiroshi [1 ]
Sivakumar, Thillaiampalam [1 ]
Boldbaatar, Damdinsuren [2 ]
Yokoyama, Naoaki [1 ]
Igarashi, Ikuo [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Kagoshima Univ, Fac Agr, Dept Frontier Vet Med, Lab Emerging Infect Dis, Kagoshima 8900065, Japan
来源：
PARASITOLOGY RESEARCH | 2009年 / 105卷 / 01期
基金：
日本学术振兴会;
关键词：
RHOPTRY-ASSOCIATED PROTEIN-1; BOVIS; ANTIBODIES; INFECTION; CATTLE; EXPRESSION; DIAGNOSIS; RESISTANCE; STATE; CELLS;
D O I：
10.1007/s00436-009-1392-2
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A truncated fragment of the gene encoding the 200-kDa protein (P200) of Babesia bigemina was cloned into a plasmid vector, pGEX-4 T-1 and expressed in Escherichia coli as a glutathione-S-transferase fused protein. An indirect enzyme-linked immunosorbent assay (ELISA) using the rp200/CT detected specific antibodies in cattle experimentally infected with B. bigemina. Furthermore, the antigen did not cross-react with antibodies to Babesia bovis, a closely related Babesia parasite indicating that rp200/CT is a specific antigen for the diagnosis of B. bigemina infection. Additionally, ELISA using p200/CT and polymerase chain reaction were conducted on serum and corresponding DNA samples obtained from field cattle to evaluate the diagnostic utility of the p200/CT antigen. Results from the current study suggest that p200/CT ELISA is a sensitive and specific method for improved serodiagnosis of B. bigemina infection.
引用
收藏
页码：249 / 254
页数：6
相关论文
共 50 条
  • [1] Evaluation of Babesia bigemina 200 kDa recombinant antigen in enzyme-linked immunosorbent assay
    Khukhuu Altangerel
    Andy Alhassan
    Hiroshi Iseki
    Thillaiampalam Sivakumar
    Damdinsuren Boldbaatar
    Naoaki Yokoyama
    Ikuo Igarashi
    Parasitology Research, 2009, 105 : 249 - 254
  • [2] Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle
    Molloy, JB
    Bowles, PM
    Jeston, PJ
    Bruyeres, AG
    Bowden, JM
    Bock, RE
    Jorgensen, WK
    Blight, GW
    Dalgliesh, RJ
    PARASITOLOGY RESEARCH, 1998, 84 (08) : 651 - 656
  • [3] Slide Enzyme-Linked Immunosorbent Assay for the Diagnosis of Babesia bigemina Infection in Bovines
    R. Ravindran
    A. K. Mishra
    J. R. Rao
    Veterinary Research Communications, 2007, 31 : 999 - 1004
  • [4] Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle
    J. B. Molloy
    P. M. Bowles
    P. J. Jeston
    A. G. Bruyeres
    J. M. Bowden
    R. E. Bock
    W. K. Jorgensen
    G. W. Blight
    R. J. Dalgliesh
    Parasitology Research, 1998, 84 : 651 - 656
  • [5] Slide enzyme-linked immunosorbent assay for the diagnosis of Babesia bigemina infection in bovines
    Ravindran, R.
    Mishra, A. K.
    Rao, J. R.
    VETERINARY RESEARCH COMMUNICATIONS, 2007, 31 (08) : 999 - 1004
  • [6] Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
    Ruiz, PMG
    Passos, LM
    Machado, RZ
    Lima, JD
    Ribeiro, MF
    MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2001, 96 (02): : 237 - 240
  • [7] Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle
    Goff, Will L.
    Johnson, Wendell C.
    Molloy, John B.
    Jorgensen, Wayne K.
    Waldron, Susan J.
    Figueroa, Julio V.
    Matthee, Olivier
    Adams, D. Scott
    McGuire, Travis C.
    Pino, Ignacio
    Mosqueda, Juan
    Palmer, Guy H.
    Suarez, Carlos E.
    Knowles, Donald P.
    McElwain, Terry F.
    CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (09) : 1316 - 1321
  • [8] Comparative evaluation of Babesia bigemina truncated C-terminal rhoptry associated protein-1 and 200 kDa merozoite protein in indirect enzyme-linked immunosorbent assay
    Shahzad, Munazah
    Garg, Rajat
    Yadav, Shobha
    Devi, Anjali
    Ram, Hira
    Banerjee, P. S.
    TICKS AND TICK-BORNE DISEASES, 2021, 12 (05)
  • [9] Evaluation of a Novel Aspergillus Antigen Enzyme-Linked Immunosorbent Assay
    Dichtl, Karl
    Seybold, Ulrich
    Ormanns, Steffen
    Horns, Heidi
    Wagener, Johannes
    JOURNAL OF CLINICAL MICROBIOLOGY, 2019, 57 (07)
  • [10] Babesia gibsoni:: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP
    Goo, Youn-Kyoung
    Jia, Honglin
    Aboge, G. Oluga
    Terkawi, M. Alaa
    Kuriki, Ken
    Nakamura, Chinatsu
    Kumagai, Akiko
    Zhou, Jinlin
    Lee, Eung-goo
    Nishikawa, Yoshifumi
    Igarashi, Ikuo
    Fujisaki, Kozo
    Xuan, Xuenan
    EXPERIMENTAL PARASITOLOGY, 2008, 118 (04) : 555 - 560
12345