Screening of Xanthine Oxidase Inhibitors in Traditional Chinese Herbal Formulae Ermiaowan Using Ultrafiltration UPLC-MSn

被引：7
|
作者：
Xu Chen [1 ,2 ]
Liu Shu [1 ]
Liu Zhiqiang [1 ]
Song Fengrui [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Ctr Mass Spectrometry Changchun, Jilin Prov Key Lab Tradit Chinese Med Chem & Mass, Changchun 130022, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2014年 / 35卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Ermiaowan; Xanthine oxidase; Ultrafiltration UPLC/Q-TOF/MS; ALPHA-GLUCOSIDASE INHIBITORS; MASS-SPECTROMETRY; FREE-RADICALS; URIC-ACID; EXTRACT; SERUM; ANTIOXIDANTS; IONIZATION; ALKALOIDS; ASSAY;
D O I：
10.7503/cjcu20140419
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An ultrafiltration UPLC/Q-TOF/MS assay and in vitro xanthine oxidase inhibition assay were combined to screen xanthine oxidase inhibitors in traditional Chinese herbal formulae Ermiaowan. The result of in vitro xanthine oxidase inhibition assay showed that Ermiaowan had a strong xanthine oxidase inhibitory activity, the IC50 value was (0.218 +/- 0.0034) mg/mL. Nine compounds were screened and identified as potential xanthine oxidase inhibitors in Ermiaowan using ultrafiltration UPLC/Q-TOF/MS. This work provides a certain basis for the development of xanthine oxidase inhibitors and the mechanism clarification of Ermiaowan to treat gout and hyperuricemia.
引用
收藏
页码：1640 / 1645
页数：6
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