M2, M3, and M4 muscarinic receptors are expressed in the guinea pig gallbladder

被引：2
|
作者：
Cabadak, Hulya [1 ]
Cuadra, Adolfo E. [2 ]
El-Fakahany, Esam E. [2 ]
Kan, Beki [1 ]
机构：
[1] Marmara Univ, Sch Med, Dept Biophys, TR-34668 Istanbul, Turkey
[2] Univ Minnesota, Sch Med, Div Neurosci Res Psychiat, Minneapolis, MN 55455 USA
来源：
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION | 2009年 / 29卷 / 01期
基金：
美国国家卫生研究院;
关键词：
Muscarinic receptors; guinea pig gallbladder; RT-PCR; MESSENGER-RNA LEVELS; SMOOTH-MUSCLE; CELLS;
D O I：
10.1080/10799890802687416
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: The identity of muscarinic acetylcholine receptors (mAchR) involved in cholinergic-mediated contraction of the guinea pig gallbladder has been a matter of debate. Different groups have suggested the involvement of M-1, M-2, M-3, or M-4 receptor subtypes in the contraction of this tissue. The objective of this study was to identify the mAchR subtypes expressed in the guinea pig gallbladder by RT-PCR. Methods: Total RNA prepared from frozen guinea pig gallbladder tissue was amplified by using specific primers for the M-1-M-4 receptor subtypes. Results: M-2, M-3, and M-4 transcripts were detected in the following rank order: M-4 > M-2 > M-3. We were unable to demonstrate the expression of the M, receptor subtype in this tissue. Conclusions: Our results are in agreement with our previous binding and functional data.
引用
收藏
页码：63 / 66
页数：4
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