Engineered CaM2 modulates nuclear calcium oscillation and enhances legume root nodule symbiosis

被引：7

作者：

del Cerro, Pablo ^{[3
]}

Cook, Nicola M. ^{[3
]}

Huisman, Rik ^{[1
,3
]}

Dangeville, Pierre ^{[3
]}

Grubb, Lauren E. ^{[3
]}

Marchal, Clemence ^{[2
,3
]}

Lam, Anson Ho Ching ^{[3
]}

Charpentier, Myriam ^{[3
]}

机构：

[1] Lab Mol Biol, NL-6708 PB Wageningen, Netherlands

[2] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

[3] John Innes Inst, Cell & Dev Biol Dept, Norwich NR4 7UH, Norfolk, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2022年 / 119卷 / 13期

基金：

英国生物技术与生命科学研究理事会; 英国科研创新办公室;

关键词：

root legume symbiosis; calcium signaling; calmodulin; NUCLEOTIDE-GATED CHANNELS; TRANSCRIPTION FACTOR; KINASE CCAMK; CALMODULIN; NODULATION; PERCEPTION; BINDING; SPIKING; RECOGNITION; ACTIVATION;

D O I：

10.1073/pnas.2200099119

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The key physiological event essential to the establishment of nitrogen -fixing bacteria and phosphate-delivering arbuscular mycorrhizal symbioses is the induction of nuclear calcium oscillations that are required for endosymbioses. These regular fluctuations in nucleoplasmic calcium concentrations are generated by ion channels and a pump located at the nuclear envelope, including the CYCLIC NUCLEOTIDE GATED CHANNEL 15 (CNGC15). However, how the CNGC15s are regulated in planta to sustain a calcium oscillatory mechanism remains unknown. Here, we demonstrate that the CNGC15s are regulated by the calcium-bound form of the calmodulin 2 (holo-CaM2), which, upon release of calcium, provides negative feedback to close the CNGC15s. Combining structural and evolutionary analyses of CaM residues with bioinformatic analysis, we engineered a holo-CaM2 with an increased affinity for CNGC15s. In planta, the expression of the engineered holo-CaM2 accelerates the calcium oscillation frequency, early endosymbioses signaling and is sufficient to sustain over time an enhanced root nodule symbiosis but not an increased arbuscular mycorrhization. Together, these results reveal that holo-CaM2 is a component of endosymbiosis signaling required to modulate CNGC15s activity and the downstream root nodule symbiosis pathway.

引用

页数：7