Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2

被引：223

作者：

Daruwala, R ^{[1
]}

Song, J ^{[1
]}

Koh, WS ^{[1
]}

Rumsey, SC ^{[1
]}

Levine, M ^{[1
]}

机构：

[1] NIDDK, Mol & Clin Nutr Sect, NIH, Bethesda, MD 20892 USA

来源：

FEBS LETTERS | 1999年 / 460卷 / 03期

关键词：

vitamin C; ascorbate; transport; hSVCT1; hSVCT2;

D O I：

10.1016/S0014-5793(99)01393-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two sodium-dependent vitamin C transporters, hSVCT1 and hSVCT2, were cloned from a human kidney cDNA library. hSVCT1 had a 1797 bp open reading frame encoding a 598 amino acid polypeptide. The 1953 bp open reading frame of hSVCT2 encoded a 650 amino acid polypeptide. Using a Xenopus laevis oocyte expression system, both transporters were functionally expressed. BS Eadie-Hofstee transformation the apparent K-m of hSVCT1 for ascorbate was 252.0 mu M and of hSVCT2 for ascorbate was 21.3 mu M. Both transporters were sodium-dependent and did not transport dehydroascorbic acid. Incubation of oocytes expressing either transporter with phorbol 12-myristate 13-acetate (PMA) inhibited ascorbate transport activity. Availability of the human transporter clones may facilitate new strategies for determining vitamin C intake. (C) 1999 Federation of European Biochemical Societies.

引用

页码：480 / 484

页数：5

共 50 条

[1] Molecular cloning and characterization of a human vitamin C transporter (hSVCT1).
Wang, Y
MacKenzie, B
Tsukaguchi, H
Tokui, T
Berger, UV
Hediger, MA
[J]. FASEB JOURNAL, 1999, 13 (04): : A374 - A374
[2] Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting
Velho, Albertina M.
Jarvis, Simon M.
[J]. EXPERIMENTAL CELL RESEARCH, 2009, 315 (13) : 2312 - 2321
[3] MicroRNA-103a plays a role in regulating human sodium-dependent vitamin C transporter-1 (hSVCT1) in intestinal epithelial cells
Subramanian, Veedamali
Sabui, Subrata
Marchant, Jonathan
Said, Hamid
[J]. FASEB JOURNAL, 2019, 33
[4] Cytosolic C-terminus sequence dictates the apical targeting of the human sodium-dependent vitamin C transporter (hSVCT1) in intestinal and renal epithelial cells
Subramanian, VS
Marchant, JS
Boulware, MJ
Said, HM
[J]. GASTROENTEROLOGY, 2004, 126 (04) : A44 - A44
[5] Both NH2 and COOH Terminals Are Essential for Cell Membrane Targeting of Human Sodium-Dependent Vitamin C Transporter-2 (hSVCT2) in Human Liver Cells
Subramanian, Veedamali S.
Marchant, Jonathan S.
Said, Hamid M.
[J]. GASTROENTEROLOGY, 2009, 136 (05) : A729 - A729
[6] Human Na+-dependent vitamin C transporter 1 (hSVCT1):: primary structure, functional characteristics and evidence for a non-functional splice variant
Wang, HP
Dutta, B
Huang, W
Devoe, LD
Leibach, FH
Ganapathy, V
Prasad, PD
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1461 (01): : 1 - 9
[7] Functional role of conserved transmembrane segment 1 residues in human sodium-dependent vitamin C transporters
Varma, Saaket
Campbell, Christine E.
Kuo, Shiu-Ming
[J]. BIOCHEMISTRY, 2008, 47 (09) : 2952 - 2960
[8] Functional expression of sodium-dependent vitamin C transporter 2 in human endothelial cells
Seno, T
Inoue, N
Matsui, K
Ejiri, J
Hirata, K
Kawashima, S
Yokoyama, M
[J]. JOURNAL OF VASCULAR RESEARCH, 2004, 41 (04) : 345 - 351
[9] Characterization of a functional bacterial homologue of sodium-dependent neurotransmitter transporters
Androutsellis-Theotokis, A
Goldberg, NR
Ueda, K
Beppu, T
Beckman, ML
Das, S
Javitch, JA
Rudnick, G
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (15) : 12703 - 12709
[10] Distribution of sodium-dependent Vitamin C transporters in adult rat brain
Lee, Juhyun
Mun, Ga hee
Kim, Myeung Ju
Kim, Hyun Jun
Chung, Yoon Hee
Chung, Young Bae
Kang, Jae Seung
Hwang, Young Il
Oh, Scung Ha
Kim, Joong-Gon
Hwang, Douk Ho
Shin, Dong Hoon
Lee, Wang Jae
[J]. JOURNAL OF IMMUNOLOGY, 2006, 176 : S30 - S30

← 1 2 3 4 5 →