Resveratrol inhibits glucose-induced migration of vascular smooth muscle cells mediated by focal adhesion kinase

被引:35
|
作者
Lin, Yi-Chiao [1 ,2 ]
Chen, Li-Hsuen [1 ,2 ]
Varadharajan, T. [1 ,2 ]
Tsai, May-Jywan [3 ]
Chia, Yi-Chen [4 ]
Yuan, Ta-Chun [1 ,2 ]
Sung, Ping-Jyun [1 ,2 ,5 ]
Weng, Ching-Feng [1 ,2 ,5 ]
机构
[1] Natl Dong Hwa Univ, Dept Life Sci, Hualien, Taiwan
[2] Natl Dong Hwa Univ, Inst Biotechnol, Hualien, Taiwan
[3] Taipei Vet Gen Hosp, Neurol Inst, Neural Regenerat Lab, Taipei, Taiwan
[4] Tajen Univ, Dept Food Sci & Technol, Pingtung, Taiwan
[5] Natl Dong Hwa Univ, Dept Life Sci, Grad Inst Marine Biotechnol, Pingtung, Taiwan
关键词
FAK; High glucose; Migration; Resveratrol; Vascular smooth muscle cell; RED WINE ANTIOXIDANT; INFLAMMATORY RESPONSE; UP-REGULATION; ACTIVATION; EXPRESSION; PROLIFERATION; LAMELLIPODIA; SECRETION; ALCOHOL; PRODUCT;
D O I
10.1002/mnfr.201300698
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Scope: Diabetes is a critical factor for atherosclerosis, as hyperglycemia induces vascular smooth muscle cell (VSMC) proliferation and migration and subsequently contributes to the formation of atherosclerotic lesions. This study investigates whether resveratrol plays a regulatory role in the proliferation and migration of VSMCs under high glucose induction to imitate a hyperglycemic condition. Methods and results: Resveratrol inhibited the migration of VSMCs in the wound-healing assay and the formation of lamellipodia and filopodia as assessed by atomic force microscopy scanning. Resveratrol suppressed the mRNA expression of c-Src, Rac1, cdc42, IRS-1, MEKK1, MEKK4, and mitogen-activated protein kinase along with the protein levels of c-Src, p-Src, and cdc42 in VSMCs. Resveratrol decreased the level of p-FAK protein under normal glucose conditions. Resveratrol could inhibit the activities of matrix metalloproteinase (MMP) 2 and MMP 9 as shown by zymography. Moreover, resveratrol also regulated the mitogen-activated protein kinase pathway and MMP activities of VSMC migration under the high glucose condition. Conclusion: The antimigratory effects of resveratrol by reduced MMP expression through the inhibition of Rac1, p-FAK, and lamellipodia formation and the activation of p-AKT and p-ERK1/2 suggest that resveratrol is a potential compound for the treatment of vascular diseases via the regulation of VSMC migration.
引用
收藏
页码:1389 / 1401
页数:13
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