Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity

被引：5

作者：

Yang, Gang ^{[1
,2
]}

Wang, Huanyu ^{[1
,2
]}

Feng, Mengyu ^{[1
,2
]}

You, Lei ^{[1
,2
]}

Zheng, Lianfang ^{[2
,3
]}

Zhang, Taiping ^{[1
,2
,4
]}

Cong, Lin ^{[1
,2
]}

Zhao, Yupei ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Gen Surg, Beijing 100730, Peoples R China

[2] Peking Union Med Coll, Beijing 100730, Peoples R China

[3] Chinese Acad Med Sci, Peking Union Med Hosp, Dept Nucl Med, Beijing 100730, Peoples R China

[4] Chinese Acad Med Sci, Clin Immunol Ctr, Beijing 100730, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2019年 / 62卷 / 06期

基金：

中国国家自然科学基金;

关键词：

pancreatic cancer; high metastatic activity; gene expression; methylation; EPITHELIAL-MESENCHYMAL TRANSITION; PERITONEAL DISSEMINATION; MODEL; PROGRESSION; ACTIVATION; CARCINOMA; STRINGTIE; REVEALS; LIVER;

D O I：

10.1007/s11427-018-9495-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized both in vitro and in vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of mRNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells, indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer.

引用

页码：791 / 806

页数：16