Preliminary Report on the Effect of Savanna Plants Leucaena leucocephala, Parkia platycephala and Senna alata against Eggs and Immature Stages of Trichostrongylid Nematodes In Vitro

被引：4

作者：

Silva Figueiredo, Benta Natania ^{[1
,2
]}

Sato, Marcello Otake ^{[3
]}

Sousa Moura, Laiane Teixeira ^{[1
]}

Botelho Mariano, Sandra Maria ^{[4
]}

Alvim, Tarso da Costa ^{[5
]}

Soares, Ilsamar Mendes ^{[5
]}

Kawai, Satoru ^{[3
]}

Ascencio, Sergio Donizeti ^{[5
]}

Santos, Helcileia Dias ^{[1
]}

Paiva, Joseilson Alves ^{[1
]}

Sato, Megumi ^{[6
]}

Maruo, Viviane Mayumi ^{[1
]}

机构：

[1] Univ Fed Tocantins, Escola Med Vet & Zootecnia, BR-77804970 Araguaina, Tocantins, Brazil

[2] Ctr Univ Catolica Tocantins, Escola Ciencias Agr & Ambientais, BR-77061002 Palmas, Tocantins, Brazil

[3] Dokkyo Med Univ, Dept Trop Med & Parasitol, Mibu, Tochigi 3210293, Japan

[4] Univ Fed Tocantins, Curso Med, BR-77001090 Palmas, Tocantins, Brazil

[5] Univ Fed Tocantins, Lab Pesquisa Prod Nat, BR-77001090 Palmas, Tocantins, Brazil

[6] Niigata Univ, Grad Sch Hlth Sci, Niigata, Niigata 9518518, Japan

来源：

PATHOGENS | 2020年 / 9卷 / 12期

关键词：

nematicide activity; parasite control; phytotherapy; plant secondary metabolites; ANTHELMINTIC ACTIVITY; HAEMONCHUS-CONTORTUS; PARASITIC NEMATODES; CONDENSED TANNINS; MEDICINAL-PLANTS; EXTRACTS; RESISTANCE; CHEMISTRY;

D O I：

10.3390/pathogens9120986

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The current study evaluated the anthelmintic effect of different extracts of Leucaena leucocephala, Parkia platycephala, and Senna alata on trichostrongylid eggs and infective larvae and determined the potential active components of each plant. Dried and macerated plant material was concentrated using rotaevaporation to obtain the crude extract (CE), followed by solvent partitioning to obtain hexanic (HexE), acetatic (AcE), and butanolic (BuE) extracts used for phytochemical analysis and anthelmintic efficacy testing in vitro. All the crude and partitioned extracts tested showed inhibition activity in the hatching of trichostrongylid eggs. Larvicidal efficacy was observed at CE concentrations of 2.5, 5.0, and 7.5 mg/mL for P. platycephala and S. alata. However, L. leucocephala CE did not significantly reduce the number of living larvae in the tested concentrations. Chromatographic analysis revealed several active metabolites; gallic acid, ellagic acid, naringin, morin, and kaempferol on AcE of P. platycephala; gallic acid, rutin, and ellagic acid on BuE of P. platycephala; and gallic acid and naringin on BuE of L. leucocephala. The extracts of P. platycephala, L. leucocephala, and S. alata leaves showed egg hatching inhibition and larvicidal activity, probably produced by tannins and flavonoids, which may act alone or by synergism.

引用

页码：1 / 10

页数：10