Oligonucleotide Array-based Detection and Genotyping of Mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma)

被引：2

作者：

Jang, Hyunjung ^{[2
]}

Kim, Hyomyeung ^{[2
]}

Kang, Byeongchul ^{[3
]}

Kim, Cheolmin ^{[2
,4
]}

Park, Heekyung ^{[1
]}

机构：

[1] PANAGENE Inc, Taejon 305510, South Korea

[2] Geneln Co Ltd, Inst Genom Med, Pusan 617030, South Korea

[3] Insilicogen Inc, Inst Support Bioinformat, Suwon 441813, South Korea

[4] Pusan Natl Univ, Sch Med, Dept Biochem, Pusan 602739, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 19卷 / 03期

关键词：

Oligonucleotide array; mollicute; internal transcribed spacer; genotyping; CELL-CULTURE; IDENTIFICATION;

D O I：

10.4014/jmb.0806.347

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An oligonucleotide array was developed to detect and genotype mollicutes based on the internal transcribed spacer (ITS) sequence. The results of the assay were compared with those of a PCR-RFLP assay. The proposed oligonucleotide array containing 5 genus- and 23 species-specific probes was able to detect Mycoplasma species, including M. penetrans and M. spermatophilum, that were not detected by the PCR-RFLP assay. Therefore, the results demonstrated that the proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products are safe and of good quality.

引用

页码：265 / 270

页数：6

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