DETECTION OF FUMONISIN PRODUCING FUSARIUM VERTICILLIOIDES IN PADDY (ORYZA SATIVA L.) USING POLYMERASE CHAIN REACTION (PCR)

被引：0

作者：

Maheshwar, P. K. ^{[2
]}

Moharram, S. Ahmed ^{[1
]}

Janardhana, G. R. ^{[1
]}

机构：

[1] Univ Mysore, Dept Studies Bot, Mysore 570006, Karnataka, India

[2] Univ Mysore, Yuvarajas Coll, Dept Microbiol, Mysore 570005, Karnataka, India

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2009年 / 40卷 / 01期

关键词：

Fusarium verticillioides; fumonisin; paddy; PCR; RICE; MONILIFORME; MYCOTOXINS; MYCOFLORA; STRAINS; MAIZE; FUNGI; ASSAY;

D O I：

10.1590/S1517-83822009000100023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin.

引用

页码：134 / 138

页数：5

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