Flow injection spectrophotometric determination of L-ascorbic acid in biological matters

被引：31
|
作者：
Kleszczewski, T
Kleszczewska, E
机构：
[1] Med Acad, Inst Biophys, PL-15230 Bialystok, Poland
[2] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 29卷 / 04期
关键词：
flow injection analysis; L-ascorbic acid; tissues;
D O I：
10.1016/S0731-7085(02)00127-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for spectrophotometric determination Of L-ascorbic acid (vitamin Q in tissues by flow injection analysis is reported. The procedure is based on the oxidation of analyse with iron(III) and 2,2'-dipyridyl. Concentrations of vitamin C in the range of 0.5-20 ppm have been determined with a relative standard deviation 1.2% (n = 15). The injection rate was 40 samples per h. The method was applied to the determination Of L-ascorbic acid in rat's tissues (blood serum, brain, and liver) and compared favourably with an independent reference method based on spectrophotometry. (C) 2002 Published by Elsevier Science B.V.
引用
收藏
页码：755 / 759
页数：5
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