Using gelatin scaffold with coated basic fibroblast growth factor as a transfer system for transplantation of human neural stem cells

被引：12

作者：

Chen, Y. -W.

Chiou, S. -H.

Wong, T. -T.

Ku, H. -H.

Lin, H. -T.

Chung, C. -F.

Yen, S. -H.

Kao, C. -L.

机构：

[1] Taipei Vet Gen Hosp, Ctr Canc, Taipei, Taiwan

[2] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan

[3] Taipei Vet Gen Hosp, Neurol Inst, Div Pediat Neurosurg, Taipei, Taiwan

[4] Natl Yang Ming Univ, Inst Anat & Cell Biol, Taipei 112, Taiwan

[5] Natl Yang Ming Univ, Inst Clin Med, Taipei 112, Taiwan

来源：

TRANSPLANTATION PROCEEDINGS | 2006年 / 38卷 / 05期

关键词：

D O I：

10.1016/j.transproceed.2006.02.084

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.

引用

页码：1616 / 1617

页数：2

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